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Title: Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells.
Authors: Ohta, Ryo
Niwa, Akira  kyouindb  KAKEN_id
Taniguchi, Yukimasa
Suzuki, Naoya M
Toga, Junko
Yagi, Emiko
Saiki, Norikazu
Nishinaka-Arai, Yoko
Okada, Chihiro
Watanabe, Akira  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-4381-4229 (unconfirmed)
Nakahata, Tatsutoshi
Sekiguchi, Kiyotoshi
Saito, Megumu K
Author's alias: 太田, 諒
丹羽, 明
中畑, 龍俊
関口, 清俊
齋藤, 潤
Issue Date: 2-Nov-2016
Publisher: Springer Nature
Journal title: Scientific reports
Volume: 6
Thesis number: 35680
Abstract: Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for their clinical application. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrate that the directed switching of defined ECMs robustly yields highly-purified (>95%) endothelial progenitor cells (PSC-EPCs) without cell sorting from hPSCs in an integrin-laminin axis-dependent manner. Single-cell RNA-seq analysis revealed that LM411-E8 resolved intercellular transcriptional heterogeneity and escorted the progenitor cells to the appropriate differentiation pathway. The PSC-EPCs gave rise to functional endothelial cells both in vivo and in vitro. We therefore propose that sequential switching of defined matrices is an important concept for guiding cells towards desired fate.
Description: 細胞外マトリックスを用いてヒト多能性幹細胞から高効率に血管内皮細胞の誘導に成功. 京都大学プレスリリース. 2016-11-09.
Rights: © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/217247
DOI(Published Version): 10.1038/srep35680
PubMed ID: 27804979
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2016-11-09
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