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タイトル: | Production of ricinoleic acid-containing monoestolide triacylglycerides in an oleaginous diatom, Chaetoceros gracilis. |
著者: | Kajikawa, Masataka https://orcid.org/0000-0003-0361-2769 (unconfirmed) Abe, Tatsuki Ifuku, Kentaro https://orcid.org/0000-0003-0241-8008 (unconfirmed) Furutani, Ken-Ichi Yan, Dongyi Okuda, Tomoyo Ando, Akinori https://orcid.org/0000-0002-8359-7441 (unconfirmed) Kishino, Shigenobu https://orcid.org/0000-0002-4587-2422 (unconfirmed) Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) Fukuzawa, Hideya https://orcid.org/0000-0003-0963-5662 (unconfirmed) |
著者名の別形: | 梶川, 昌孝 伊福, 健太郎 安藤, 晃規 岸野, 重信 小川, 順 福澤, 秀哉 |
発行日: | 10-Nov-2016 |
出版者: | Springer Nature |
誌名: | Scientific reports |
巻: | 6 |
論文番号: | 36809 |
抄録: | Ricinoleic acid (RA), a hydroxyl fatty acid, is suitable for medical and industrial uses and is produced in high-oil-accumulating organisms such as castor bean and the ergot fungus Claviceps. We report here the efficient production of RA in a transgenic diatom Chaetoceros gracilis expressing the fatty acid hydroxylase gene (CpFAH) from Claviceps purpurea. In transgenic C. gracilis, RA content increased at low temperatures, reaching 2.2 pg/cell when cultured for 7 d at 15 °C, without affecting cell growth, and was enhanced (3.3 pg/cell) by the co-expression of a palmitic acid-specific elongase gene. Most of the accumulated RA was linked with monoestolide triacylglycerol (ME TAG), in which one RA molecule was esterified to the α position of the glycerol backbone and was further esterified at its hydroxy group with a fatty acid or second RA moiety, or 1-OH TAG, in which RA was esterified to the glycerol backbone. Overall, 80% of RA was accumulated as ME TAGs. Furthermore, exogenous RA-methyl ester suppressed the growth of wild-type diatoms in a dose-dependent manner and was rapidly converted to ME TAG. These results suggest that C. gracilis masks the hydroxyl group and accumulates RA as the less-toxic ME TAG. |
記述: | 実用珪藻ツノケイソウによるリシノール酸の生産に成功. 京都大学プレスリリース. 2016-11-14. |
著作権等: | © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
URI: | http://hdl.handle.net/2433/217256 |
DOI(出版社版): | 10.1038/srep36809 |
PubMed ID: | 27830762 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2016-11-14 |
出現コレクション: | 学術雑誌掲載論文等 |
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