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Title: Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser.
Authors: Tono, Kensuke
Nango, Eriko  kyouindb  KAKEN_id
Sugahara, Michihiro
Song, Changyong
Park, Jaehyun
Tanaka, Tomoyuki
Tanaka, Rie
Joti, Yasumasa
Kameshima, Takashi
Ono, Shun
Hatsui, Takaki
Mizohata, Eiichi
Suzuki, Mamoru
Shimamura, Tatsuro  kyouindb  KAKEN_id
Tanaka, Yoshiki
Iwata, So  kyouindb  KAKEN_id
Yabashi, Makina
Author's alias: 島村, 達郎
Keywords: serial femtosecond crystallography
Issue Date: May-2015
Publisher: International Union of Crystallography
Journal title: Journal of synchrotron radiation
Volume: 22
Issue: Pt. 3
Start page: 532
End page: 537
Abstract: An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1 atm. The ambient pressure operation facilitates applications to fluid samples. Three kinds of injectors are employed to feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme crystals were performed by using the 10 keV XFEL of the SPring-8 Angstrom Compact free-electron LAser (SACLA). The structure of model protein lysozyme from 1 µm crystals at a resolution of 2.4 Å was obtained.
Rights: This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
DOI(Published Version): 10.1107/S1600577515004464
PubMed ID: 25931065
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