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タイトル: The efficacy of a scaffold-free Bio 3D conduit developed from human fibroblasts on peripheral nerve regeneration in a rat sciatic nerve model
著者: Yurie, Hirofumi
Ikeguchi, Ryosuke
Aoyama, Tomoki  kyouindb  KAKEN_id
Kaizawa, Yukitoshi
Tajino, Junichi
Ito, Akira  kyouindb  KAKEN_id
Ohta, Souichi
Oda, Hiroki
Takeuchi, Hisataka
Akieda, Shizuka
Tsuji, Manami
Nakayama, Koichi
Matsuda, Shuichi  kyouindb  KAKEN_id
著者名の別形: 池口, 良輔
青山, 朋樹
中山, 功一
松田, 秀一
発行日: 13-Feb-2017
出版者: Public Library of Science
誌名: PLOS ONE
巻: 12
号: 2
論文番号: e0171448
抄録: Background: Although autologous nerve grafting is the gold standard treatment of peripheral nerve injuries, several alternative methods have been developed, including nerve conduits that use supportive cells. However, the seeding efficacy and viability of supportive cells injected in nerve grafts remain unclear. Here, we focused on a novel completely biological, tissue-engineered, scaffold-free conduit. Methods: We developed six scaffold-free conduits from human normal dermal fibroblasts using a Bio 3D Printer. Twelve adult male rats with immune deficiency underwent mid-thigh-level transection of the right sciatic nerve. The resulting 5-mm nerve gap was bridged using 8-mm Bio 3D conduits (Bio 3D group, n = 6) and silicone tube (silicone group, n = 6). Several assessments were conducted to examine nerve regeneration eight weeks post-surgery. Results: Kinematic analysis revealed that the toe angle to the metatarsal bone at the final segment of the swing phase was significantly higher in the Bio 3D group than the silicone group (-35.78 ± 10.68 versus -62.48 ± 6.15, respectively; p < 0.01). Electrophysiological studies revealed significantly higher compound muscle action potential in the Bio 3D group than the silicone group (53.60 ± 26.36% versus 2.93 ± 1.84%; p < 0.01). Histological and morphological studies revealed neural cell expression in all regions of the regenerated nerves and the presence of many well-myelinated axons in the Bio 3D group. The wet muscle weight of the tibialis anterior muscle was significantly higher in the Bio 3D group than the silicone group (0.544 ± 0.063 versus 0.396 ± 0.031, respectively; p < 0.01). Conclusions: We confirmed that scaffold-free Bio 3D conduits composed entirely of fibroblast cells promote nerve regeneration in a rat sciatic nerve model.
記述: バイオ3Dプリンターを用いた末梢神経損傷に対する神経再生技術の開発. 京都大学プレスリリース. 2017-02-27.
著作権等: © 2017 Yurie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/218464
DOI(出版社版): 10.1371/journal.pone.0171448
PubMed ID: 28192527
関連リンク: http://www.kyoto-u.ac.jp/ja/research/research_results/2016/170213_1.html
出現コレクション:学術雑誌掲載論文等

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