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Title: Cell Therapy Using Human Induced Pluripotent Stem Cell-Derived Renal Progenitors Ameliorates Acute Kidney Injury in Mice
Authors: Toyohara, Takafumi
Mae, Shin-Ichi  kyouindb  KAKEN_id
Sueta, Shin-Ichi
Inoue, Tatsuyuki
Yamagishi, Yukiko
Kawamoto, Tatsuya
Kasahara, Tomoko
Hoshina, Azusa
Toyoda, Taro  kyouindb  KAKEN_id  orcid (unconfirmed)
Tanaka, Hiromi
Araoka, Toshikazu
Sato-Otsubo, Aiko
Takahashi, Kazutoshi
Sato, Yasunori
Yamaji, Noboru
Ogawa, Seishi  kyouindb  KAKEN_id
Yamanaka, Shinya  kyouindb  KAKEN_id
Osafune, Kenji  kyouindb  KAKEN_id
Author's alias: 豊原, 敬文
前, 伸一
豊田, 太郎
長船, 健二
Keywords: Nephrons
Cell- and tissue-based therapy
Induced pluripotent stem cells
Acute kidney injury
SIX2 protein
Renal progenitors
Issue Date: Sep-2015
Publisher: Wiley-Blackwell
Journal title: Stem cells translational medicine
Volume: 4
Issue: 9
Start page: 980
End page: 992
Abstract: Acute kidney injury (AKI) is defined as a rapid loss of renal function resulting from various etiologies, with a mortality rate exceeding 60% among intensive care patients. Because conventional treatments have failed to alleviate this condition, the development of regenerative therapies using human induced pluripotent stem cells (hiPSCs) presents a promising new therapeutic option for AKI. We describe our methodology for generating renal progenitors from hiPSCs that show potential in ameliorating AKI. We established a multistep differentiation protocol for inducing hiPSCs into OSR1+SIX2+ renal progenitors capable of reconstituting three-dimensional proximal renal tubule-like structures in vitro and in vivo. Moreover, we found that renal subcapsular transplantation of hiPSC-derived renal progenitors ameliorated the AKI in mice induced by ischemia/reperfusion injury, significantly suppressing the elevation of blood urea nitrogen and serum creatinine levels and attenuating histopathological changes, such as tubular necrosis, tubule dilatation with casts, and interstitial fibrosis. To our knowledge, few reports demonstrating the therapeutic efficacy of cell therapy with renal lineage cells generated from hiPSCs have been published. Our results suggest that regenerative medicine strategies for kidney diseases could be developed using hiPSC-derived renal cells.
Description: ヒトiPS細胞由来の腎前駆細胞を使った細胞移植で急性腎障害(急性腎不全)のマウスに効果. 京都大学プレスリリース. 2016-07-22.
Rights: © AlphaMed Press. This article is made available under the terms of the Creative Commons Attribution License.
DOI(Published Version): 10.5966/sctm.2014-0219
PubMed ID: 26198166
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