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Title: Epidemiological Surveillance of Lymphocryptovirus Infection in Wild Bonobos
Authors: Yoshida, Tomoyuki
Takemoto, Hiroyuki  kyouindb  KAKEN_id
Sakamaki, Tetsuya
Tokuyama, Nahoko
Hart, John
Hart, Terese
Dupain, Jef
Cobden, Amy
Mulavwa, Mbangi
Kawamoto, Yoshi
Kaneko, Akihisa
Enomoto, Yuki
Sato, Eiji
Kooriyama, Takanori
Miyabe-Nishiwaki, Takako  kyouindb  KAKEN_id
Suzuki, Juri  kyouindb  KAKEN_id
Saito, Akatsuki
Okamoto, Munehiro
Tomonaga, Masaki
Matsuzawa, Tetsuro
Furuichi, Takeshi
Akari, Hirofumi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-2166-6015 (unconfirmed)
Author's alias: 吉田, 友教
明里, 宏文
Keywords: bonobo
lymphocryptovirus
epidemiology
surveillance
feces
apes
Issue Date: 12-Aug-2016
Publisher: Frontiers Media SA
Journal title: Frontiers in Microbiology
Volume: 7
Thesis number: 1262
Abstract: Lymphocryptovirus (LCV) is one of the major gena in the herpesvirus family and is widely disseminated among primates. LCVs of human and rhesus macaques are shown to be causative agents of a number of malignant diseases including lymphoma and carcinoma. Bonobos (Pan paniscus) are highly endangered and the least studied species of the great apes. Considering the potential pathogenicity of the LCV that might threaten the fate of wild bonobos, population-based epidemiological information in terms of LCV prevalence in different location of Bonobo’s habitats will help propose improved conservation strategies for the bonobos. However, such data are not available yet because it is very difficult to collect blood samples in the wild and thus virtually impossible to conduct sero-epidemiological study on the wild ape. In order to overcome this issue, we focused on evaluating anti-LCV IgA in the feces of bonobos, which are available in a non-invasive manner. Preliminary study showed that anti-LCV IgA but not IgG was efficiently and reproducibly detected in the feces of captive chimpanzees. It is noteworthy that the fecal IgA-positive individuals were seropositive for both anti-LCV IgG and IgA and that the IgA antibodies in both sera and feces were also detectable by Western blotting assay. These results indicate that the detection of fecal anti-LCV IgA is likely a reliable and feasible for epidemiological surveillance of LCV prevalence in the great apes. We then applied this method and found that 31% of wild bonobos tested were positive for anti-LCV IgA antibody in the feces. Notably, the positivity rates varied extensively among their sampled populations. In conclusion, our results in this study demonstrate that LCV is highly disseminated among wild bonobos while the prevalence is remarkably diverse in their population-dependent manner.
Rights: © 2016 Yoshida, Takemoto, Sakamaki, Tokuyama, Hart, Hart, Dupain, Cobden, Mulavwa, Kawamoto, Kaneko, Enomoto, Sato, Kooriyama, Miyabe-Nishiwaki, Suzuki, Saito, Okamoto, Tomonaga, Matsuzawa, Furuichi and Akari. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
URI: http://hdl.handle.net/2433/218526
DOI(Published Version): 10.3389/fmicb.2016.01262
PubMed ID: 27570523
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