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タイトル: | Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres |
著者: | Yamada, Toshimichi Yoshimura, Hideaki Shimada, Rintaro Hattori, Mitsuru Eguchi, Masatoshi Fujiwara, Takahiro K. Kusumi, Akihiro Ozawa, Takeaki |
著者名の別形: | 楠見, 明弘 |
発行日: | 13-Dec-2016 |
出版者: | Springer Nature |
誌名: | Scientific Reports |
巻: | 6 |
論文番号: | 38910 |
抄録: | Telomeric repeat-containing RNA (TERRA) controls the structure and length of telomeres through interactions with numerous telomere-binding proteins. However, little is known about the mechanism by which TERRA regulates the accessibility of the proteins to telomeres, mainly because of the lack of spatiotemporal information of TERRA and its-interacting proteins. We developed a fluorescent probe to visualize endogenous TERRA to investigate its dynamics in living cells. Single-particle fluorescence imaging revealed that TERRA accumulated in a telomere-neighboring region and trapped diffusive heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1), thereby inhibiting hnRNPA1 localization to the telomere. These results suggest that TERRA regulates binding of hnRNPA1 to the telomere in a region surrounding the telomere, leading to a deeper understanding of the mechanism of TERRA function. |
著作権等: | © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
URI: | http://hdl.handle.net/2433/218796 |
DOI(出版社版): | 10.1038/srep38910 |
PubMed ID: | 27958374 |
出現コレクション: | 学術雑誌掲載論文等 |
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