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タイトル: | A rat long-lasting cystitis model induced by intravesical injection of hydrogen peroxide |
著者: | Dogishi, Koji Okamoto, Ken Majima, Tsuyoshi Konishi-Shiotsu, Shizuka Homan, Takashi Kodera, Mizuki Oyama, Shohei Oyama, Tatsuya Shirakawa, Hisashi ![]() ![]() ![]() Yoshimura, Naoki Nakagawa, Takayuki ![]() ![]() Kaneko, Shuji ![]() ![]() ![]() |
著者名の別形: | 白川, 久志 中川, 貴之 金子, 周司 |
キーワード: | Chronic cystitis hydrogen peroxide hypersensitivity of bladder nerve remodeling |
発行日: | 1-Feb-2017 |
出版者: | American Physiological Society |
誌名: | Physiological Reports |
巻: | 5 |
号: | 4 |
論文番号: | e13127 |
抄録: | Novel longer lasting inflammatory bladder animal models are needed to better understand the pathophysiology of chronic cystitis. We previously developed a relatively long-lasting mouse cystitis model by intravesical injection of hydrogen peroxide (H2O2). To further evaluate its pathophysiology, in this study, we established and analyzed a rat cystitis model. Under anesthesia, 1.5% H2O2 solution was introduced transurethrally into the bladder of female rats, and kept for 30 min. The H2O2 injection significantly increased the number of micturition events up to day 14 and decreased urine volume per micturition, with the smallest volumes on day 3, compared with the vehicle-treated group. Cystometric analysis on day 7 revealed that intercontraction intervals were significantly shortened without affecting the baseline, threshold, or maximum pressures. Intravesical resiniferatoxin-evoked nociceptive behaviors, such as freezing, were significantly enhanced on days 7 and 14. Furthermore, histopathology revealed hemorrhage, edema, infiltration of neutrophils into the lamina propria, and urothelial denudation in the early phase (day 1). These damages were gradually repaired, while hyperplasia of the urothelium, vascularization, increases in fibroblast counts, and infiltration of mast cells and eosinophils were observed through the later phase (days 7 and 14). These results suggest that intravesical H2O2 injection induces relatively long-lasting cystitis with enhanced bladder activity and pain sensation in rats. This approach thus provides a novel rat long-lasting cystitis model that allows us to analyze detailed symptoms and pathophysiology of H2O2-induced cystitis model than the mouse model and may be used to investigate the pathophysiology and treatment of chronic bladder hypersensitive disorders, such as bladder pain syndrome/interstitial cystitis. |
著作権等: | a 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/218799 |
DOI(出版社版): | 10.14814/phy2.13127 |
PubMed ID: | 28242819 |
出現コレクション: | 学術雑誌掲載論文等 |

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