Downloads: 143

Files in This Item:
File Description SizeFormat 
journal.pone.0172798.pdf3.15 MBAdobe PDFView/Open
Full metadata record
DC FieldValueLanguage
dc.contributor.authorIzuhara, Masayasuja
dc.contributor.authorKuwabara, Yasuhideja
dc.contributor.authorSaito, Naritatsuja
dc.contributor.authorYamamoto, Erikaja
dc.contributor.authorHakuno, Daihikoja
dc.contributor.authorNakashima, Yasuhiroja
dc.contributor.authorHorie, Takahiroja
dc.contributor.authorBaba, Osamuja
dc.contributor.authorNishiga, Masatakaja
dc.contributor.authorNakao, Tetsushija
dc.contributor.authorNishino, Tomohiroja
dc.contributor.authorNakazeki, Fumikoja
dc.contributor.authorIde, Yuyaja
dc.contributor.authorKimura, Masahiroja
dc.contributor.authorKimura, Takeshija
dc.contributor.authorOno, Kohja
dc.contributor.alternative桑原, 康秀ja
dc.contributor.alternative齋藤, 成達ja
dc.contributor.alternative中島, 康弘ja
dc.contributor.alternative堀江, 貴裕ja
dc.date.accessioned2017-03-17T04:53:47Z-
dc.date.available2017-03-17T04:53:47Z-
dc.date.issued2017-03-02ja
dc.identifier.issn1932-6203ja
dc.identifier.urihttp://hdl.handle.net/2433/218961-
dc.description.abstractBackground: Despite recent progress with drug-eluting stents, restenosis and thrombosis after endovascular intervention are still major limitations in the treatment of cardiovascular diseases. These problems are possibly caused by inappropriate inhibition of neointimal formation and retardation of re-endothelialization on the surface of the stents. miR-126 has been shown to have the potential to enhance vascular endothelial cell proliferation. Methods and results: We designed and constructed a 27-nt double strand RNA (dsRNA) conjugated to cholesterol, which has high membrane permeability, and formed mature miR-126 after transfection. For site-specific induction of miR-126, we utilized poly (DL-lactide-co-glycolide) nanoparticles (NPs). miR-126-dsRNA-containing NPs (miR-126 NPs) significantly reduced the protein expression of a previously identified miR-126 target, SPRED1, in human umbilical vascular endothelial cells (HUVECs), and miR-126 NPs enhanced the proliferation and migration of HUVECs. On the other hand, miR-126 NPs reduced the proliferation and migration of vascular smooth muscle cells, via the suppression of IRS-1. Finally, we developed a stent system that eluted miR-126. This delivery system exhibited significant inhibition of neointimal formation in a rabbit model of restenosis. Conclusions: miR-126 NP-conjugated stents significantly inhibited the development of neointimal hyperplasia in rabbits. The present study may indicate the possibility of a novel therapeutic option to prevent restenosis after angioplasty.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherPublic Library of Scienceja
dc.rights© 2017 Izuhara et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ja
dc.titlePrevention of neointimal formation using miRNA-126-containing nanoparticle-conjugated stents in a rabbit modelja
dc.type.niitypeJournal Articleja
dc.identifier.jtitlePLOS ONEja
dc.identifier.volume12ja
dc.identifier.issue3ja
dc.relation.doi10.1371/journal.pone.0172798ja
dc.textversionpublisherja
dc.identifier.artnume0172798ja
dc.identifier.pmid28253326ja
Appears in Collections:Journal Articles

Show simple item record

Export to RefWorks


Export Format: 


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.