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Title: Dissecting in vivo steady-state dynamics of karyopherin-dependent nuclear transport
Authors: Lolodi, Ogheneochukome
Yamazaki, Hiroya
Otsuka, Shotaro
Kumeta, Masahiro  kyouindb  KAKEN_id
Yoshimura, Shige H.
Author's alias: 粂田, 昌宏
吉村, 成弘
Keywords: Cell Biology
Molecular Biology
Issue Date: 1-Jan-2016
Publisher: American Society for Cell Biology
Journal title: Molecular Biology of the Cell
Volume: 27
Issue: 1
Start page: 167
End page: 176
Abstract: Karyopherin-dependent molecular transport through the nuclear pore complex is maintained by constant recycling pathways of karyopherins coupled with the Ran-dependent cargo catch-and-release mechanism. Although many studies have revealed the bidirectional dynamics of karyopherins, the entire kinetics of the steady-state dynamics of karyopherin and cargo is still not fully understood. In this study, we used fluorescence recovery after photobleaching and fluorescence loss in photobleaching on live cells to provide convincing in vivo proof that karyopherin-mediated nucleocytoplasmic transport of cargoes is bidirectional. Continuous photobleaching of the cytoplasm of live cells expressing NLS cargoes led to progressive decrease of nuclear fluorescence signals. In addition, experimentally obtained kinetic parameters of karyopherin complexes were used to establish a kinetic model to explain the entire cargo import and export transport cycles facilitated by importin β. The results strongly indicate that constant shuttling of karyopherins, either free or bound to cargo, ensures proper balancing of nucleocytoplasmic distribution of cargoes and establishes effective regulation of cargo dynamics by RanGTP.
Rights: © 2016 Lolodi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®, ” “The American Society for Cell Biology®, ” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
URI: http://hdl.handle.net/2433/218972
DOI(Published Version): 10.1091/mbc.E15-08-0601
PubMed ID: 26538027
Appears in Collections:Journal Articles

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