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タイトル: Precise genome-wide base editing by the CRISPR Nickase system in yeast
著者: Satomura, Atsushi
Nishioka, Ryosuke
Mori, Hitoshi
Sato, Kosuke
Kuroda, Kouichi
Ueda, Mitsuyoshi
著者名の別形: 里村, 淳
黒田, 浩一
植田, 充美
発行日: 18-May-2017
出版者: Springer Nature
誌名: Scientific Reports
巻: 7
論文番号: 2095
抄録: The CRISPR/Cas9 system has been applied to efficient genome editing in many eukaryotic cells. However, the bases that can be edited by this system have been limited to those within the protospacer adjacent motif (PAM) and guide RNA-targeting sequences. In this study, we developed a genome-wide base editing technology, “CRISPR Nickase system” that utilizes a single Cas9 nickase. This system was free from the limitation of editable bases that was observed in the CRISPR/Cas9 system, and was able to precisely edit bases up to 53 bp from the nicking site. In addition, this system showed no off-target editing, in contrast to the CRISPR/Cas9 system. Coupling the CRISPR Nickase system with yeast gap repair cloning enabled the construction of yeast mutants within only five days. The CRISPR Nickase system provides a versatile and powerful technology for rapid, site-specific, and precise base editing in yeast.
記述: ゲノムのほぼ全域で正確に編集できる新ゲノム編集法の開発--ゲノムSNPsの自在編集を可能に--. 京都大学プレスリリース. 2017-05-18.
著作権等: © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/224892
DOI(出版社版): 10.1038/s41598-017-02013-7
関連リンク: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/170518_1.html
出現コレクション:学術雑誌掲載論文等

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