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Title: Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: A potential novel strategy to enhance zinc absorption by ZIP4 targeting
Authors: Hashimoto, Ayako
Ohkura, Katsuma
Takahashi, Masakazu
Kizu, Kumiko
Narita, Hiroshi
Enomoto, Shuichi
Miyamae, Yusaku
Masuda, Seiji  kyouindb  KAKEN_id
Nagao, Masaya  kyouindb  KAKEN_id
Irie, Kazuhiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7109-8568 (unconfirmed)
Ohigashi, Hajime
Andrews, Glen K.
Kambe, Taiho  kyouindb  KAKEN_id
Author's alias: 増田, 誠司
入江, 一浩
神戸, 大朋
Keywords: apical membrane
degradation
endocytosis
screening
soyasaponin Bb
zinc transporter
Issue Date: Dec-2015
Publisher: Portland Press
Journal title: Biochemical Journal
Volume: 472
Issue: 2
Start page: 183
End page: 193
Abstract: Dietary zinc deficiency puts human health at risk, so we explored strategies for enhancing zinc absorption. In the small intestine, the zinc transporter ZIP4 functions as an essential component of zinc absorption. Overexpression of ZIP4 protein increases zinc uptake and thereby cellular zinc levels, suggesting that food components with the ability to increase ZIP4 could potentially enhance zinc absorption via the intestine. In the present study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components that can increase the abundance of ZIP4. Using this ZIP4-targeting strategy, two such soybean extracts were identified that were specifically able to decrease mZip4 endocytosis in response to zinc. These soybean extracts also effectively increased the abundance of apically localized mZip4 in transfected polarized Caco2 and Madin-Darby canine kidney cells and, moreover, two apically localized mZip4 acrodermatitis enteropathica mutants. Soybean components were purified from one extract and soyasaponin Bb was identified as an active component that increased bothmZip4 protein abundance and zinc levels in Hepa cells. Finally, we confirmed that soyasaponin Bb is capable of enhancing cell surface endogenous human ZIP4 in human cells.Our results suggest that ZIP4 targetingmay represent a new strategy to improve zinc absorption in humans.
Rights: This is the accepted version of the article, which has been published in final form at http://doi.org/10.1042/BJ20150862.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/224959
DOI(Published Version): 10.1042/BJ20150862
Appears in Collections:Journal Articles

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