Access count of this item: 194
|Title:||Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: A potential novel strategy to enhance zinc absorption by ZIP4 targeting|
Irie, Kazuhiro https://orcid.org/0000-0001-7109-8568 (unconfirmed)
Andrews, Glen K.
|Author's alias:||増田, 誠司|
|Journal title:||Biochemical Journal|
|Abstract:||Dietary zinc deficiency puts human health at risk, so we explored strategies for enhancing zinc absorption. In the small intestine, the zinc transporter ZIP4 functions as an essential component of zinc absorption. Overexpression of ZIP4 protein increases zinc uptake and thereby cellular zinc levels, suggesting that food components with the ability to increase ZIP4 could potentially enhance zinc absorption via the intestine. In the present study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components that can increase the abundance of ZIP4. Using this ZIP4-targeting strategy, two such soybean extracts were identified that were specifically able to decrease mZip4 endocytosis in response to zinc. These soybean extracts also effectively increased the abundance of apically localized mZip4 in transfected polarized Caco2 and Madin-Darby canine kidney cells and, moreover, two apically localized mZip4 acrodermatitis enteropathica mutants. Soybean components were purified from one extract and soyasaponin Bb was identified as an active component that increased bothmZip4 protein abundance and zinc levels in Hepa cells. Finally, we confirmed that soyasaponin Bb is capable of enhancing cell surface endogenous human ZIP4 in human cells.Our results suggest that ZIP4 targetingmay represent a new strategy to improve zinc absorption in humans.|
|Rights:||This is the accepted version of the article, which has been published in final form at http://doi.org/10.1042/BJ20150862.|
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
|Appears in Collections:||Journal Articles |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.