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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| zeb.2014.1032.pdf | 412 kB | Adobe PDF | 見る/開く |
| タイトル: | A Novel Method for Rearing Zebrafish by Using Freshwater Rotifers (Brachionus calyciflorus) |
| 著者: | Aoyama, Yuta Moriya, Natsumi Tanaka, Shingo Taniguchi, Tomoko Hosokawa, Hiroshi   Maegawa, Shingo |
| 著者名の別形: | 細川, 浩 前川, 真吾 |
| キーワード: | Developmental Biology Animal Science and Zoology |
| 発行日: | 29-Jul-2015 |
| 出版者: | Mary Ann Liebert Inc |
| 誌名: | Zebrafish |
| 巻: | 12 |
| 号: | 4 |
| 開始ページ: | 288 |
| 終了ページ: | 295 |
| 抄録: | The zebrafish (Danio rerio) has become a powerful model organism for studying developmental processes and genetic diseases. However, there remain several problems in previous rearing methods. In this study, we demonstrate a novel method for rearing zebrafish larvae by using a new first food, freshwater rotifers (Brachionus calyciflorus). Feeding experiments indicated that freshwater rotifers are suitable as the first food for newly hatched larval fish. In addition, we revisited and improved a feeding schedule from 5 to 40 days postfertilization. Our feeding method using freshwater rotifers accelerated larval growth. At 49 dpf, one pair out of 10 pairs successfully produced six fertilized eggs. At 56, 63, and 71 dpf, 6 out of the 10 pairs constantly produced normal embryos. Our method will improve the husbandry of the zebrafish. |
| 著作権等: | Final publication is available from Mary Ann Liebert, Inc., publishers http://doi.org/10.1089/zeb.2014.1032 The full-text file will be made open to the public on 29 July 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
| URI: | http://hdl.handle.net/2433/225120 |
| DOI(出版社版): | 10.1089/zeb.2014.1032 |
| PubMed ID: | 25938499 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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