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Title: A Novel Method for Rearing Zebrafish by Using Freshwater Rotifers (Brachionus calyciflorus)
Authors: Aoyama, Yuta
Moriya, Natsumi
Tanaka, Shingo
Taniguchi, Tomoko
Hosokawa, Hiroshi  kyouindb  KAKEN_id
Maegawa, Shingo  kyouindb  KAKEN_id
Author's alias: 細川, 浩
前川, 真吾
Keywords: Developmental Biology
Animal Science and Zoology
Issue Date: 29-Jul-2015
Publisher: Mary Ann Liebert Inc
Journal title: Zebrafish
Volume: 12
Issue: 4
Start page: 288
End page: 295
Abstract: The zebrafish (Danio rerio) has become a powerful model organism for studying developmental processes and genetic diseases. However, there remain several problems in previous rearing methods. In this study, we demonstrate a novel method for rearing zebrafish larvae by using a new first food, freshwater rotifers (Brachionus calyciflorus). Feeding experiments indicated that freshwater rotifers are suitable as the first food for newly hatched larval fish. In addition, we revisited and improved a feeding schedule from 5 to 40 days postfertilization. Our feeding method using freshwater rotifers accelerated larval growth. At 49 dpf, one pair out of 10 pairs successfully produced six fertilized eggs. At 56, 63, and 71 dpf, 6 out of the 10 pairs constantly produced normal embryos. Our method will improve the husbandry of the zebrafish.
Rights: Final publication is available from Mary Ann Liebert, Inc., publishers
The full-text file will be made open to the public on 29 July 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1089/zeb.2014.1032
PubMed ID: 25938499
Appears in Collections:Journal Articles

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