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dc.contributor.author | Goodison, Steve | en |
dc.contributor.author | Ogawa, Osamu | en |
dc.contributor.author | Matsui, Yoshiyuki | en |
dc.contributor.author | Kobayashi, Takashi | en |
dc.contributor.author | Miyake, Makito | en |
dc.contributor.author | Ohnishi, Sayuri | en |
dc.contributor.author | Fujimoto, Kiyohide | en |
dc.contributor.author | Dai, Yunfeng | en |
dc.contributor.author | Shimizu, Yoshiko | en |
dc.contributor.author | Tsukikawa, Kazue | en |
dc.contributor.author | Furuya, Hideki | en |
dc.contributor.author | Rosser, Charles J. | en |
dc.contributor.alternative | 小川, 修 | ja |
dc.contributor.alternative | 松井, 喜之 | ja |
dc.contributor.alternative | 小林, 恭 | ja |
dc.date.accessioned | 2017-06-30T05:40:52Z | - |
dc.date.available | 2017-06-30T05:40:52Z | - |
dc.date.issued | 2016-10-07 | - |
dc.identifier.issn | 1479-5876 | - |
dc.identifier.uri | http://hdl.handle.net/2433/226288 | - |
dc.description.abstract | Background;; Bladder cancer (BCa) is among the most commonly diagnosed malignancies worldwide, and due the high rate of post-operative disease recurrence, it is one of the most prevalent in many countries. The development of non-invasive molecular assays that can accurately detect and monitor BCa would be a major advance, benefiting both patients and healthcare systems. We have previously identified a urinary protein biomarker panel that is being developed for application in at-risk patient cohorts. Here, we investigated the potential utility of the multiplex assay in a Japanese cohort. Methods;; The Japanese study cohort collected from urology clinics at two institutions was comprised of a total of 288 subjects. The protein biomarker panel (IL8, MMP9, MMP10, ANG, APOE, SDC1, A1AT, PAI1, CA9, VEGFA) was monitored in voided urine samples collected prior to cystoscopy using a custom multiplex ELISA assay. The diagnostic performance of the biomarker panel was assessed using receiver operator curves, predictive modeling and descriptive statistics. Results;; Urinary biomarker concentrations were significantly elevated in cases versus controls, and in cases with high-grade and muscle-invasive tumors. The AUC for the 10-biomarker assay was 0.892 (95 % confidence interval 0.850–0.934), with an overall diagnostic sensitivity specificity of 0.85 and 0.81, respectively. A predictive model trained on the larger institutional cohort correctly identified 99 % of the cases from the second institution. Conclusions;; Urinary levels of a 10-biomarker panel enabled discrimination of patients with BCa. The multiplex urinary diagnostic assay has the potential to be developed for the non-invasive detection of BCa in at-risk Japanese patients. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | © 2016 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | en |
dc.subject | Biomarkers | en |
dc.subject | Bladder cancer | en |
dc.subject | Multiplex | en |
dc.subject | Protein | en |
dc.subject | Urine | en |
dc.title | A multiplex urinary immunoassay for bladder cancer detection: analysis of a Japanese cohort | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Journal of Translational Medicine | en |
dc.identifier.volume | 14 | - |
dc.relation.doi | 10.1186/s12967-016-1043-1 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 287 | - |
dc.identifier.pmid | 27717367 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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