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j.stemcr.2017.05.003.pdf2.57 MBAdobe PDF見る/開く
タイトル: A RHO Small GTPase Regulator ABR Secures Mitotic Fidelity in Human Embryonic Stem Cells
著者: Ohgushi, Masatoshi
Minaguchi, Maki
Eiraku, Mototsugu
Sasai, Yoshiki
著者名の別形: 永樂, 元次
キーワード: human embryonic stem cells
mitotic fidelity
aneuploidy
RHO family small GTPases
ABR
cell-cell communication
発行日: 11-Jul-2017
出版者: Elsevier BV
誌名: Stem Cell Reports
巻: 9
号: 1
開始ページ: 58
終了ページ: 66
抄録: Pluripotent stem cells can undergo repeated self-renewal while retaining genetic integrity, but they occasionally acquire aneuploidy during long-term culture, which is a practical obstacle for medical applications of human pluripotent stem cells. In this study, we explored the biological roles of ABR, a regulator of RHO family small GTPases, and found that it has pivotal roles during mitotic processes in human embryonic stem cells (hESCs). Although ABR has been shown to be involved in dissociation-induced hESC apoptosis, it does not appear to have direct effects on cell survival unless cell-cell contact is impaired. Instead, we found that it is important for faithful hESC division. Mechanistically, ABR depletion compromised centrosome dynamics and predisposed the cell to chromosome misalignment and missegregation, which raised the frequency of aneuploidy. These results provide insights into the mechanisms that support the genetic integrity of self-renewing hESCs.
著作権等: © 2017 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/2433/226437
DOI(出版社版): 10.1016/j.stemcr.2017.05.003
出現コレクション:学術雑誌掲載論文等

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