Downloads: 82

Files in This Item:
File Description SizeFormat 
j.stemcr.2017.05.003.pdf2.57 MBAdobe PDFView/Open
Title: A RHO Small GTPase Regulator ABR Secures Mitotic Fidelity in Human Embryonic Stem Cells
Authors: Ohgushi, Masatoshi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-1293-4893 (unconfirmed)
Minaguchi, Maki
Eiraku, Mototsugu  kyouindb  KAKEN_id
Sasai, Yoshiki
Author's alias: 永樂, 元次
Keywords: human embryonic stem cells
mitotic fidelity
aneuploidy
RHO family small GTPases
ABR
cell-cell communication
Issue Date: 11-Jul-2017
Publisher: Elsevier BV
Journal title: Stem Cell Reports
Volume: 9
Issue: 1
Start page: 58
End page: 66
Abstract: Pluripotent stem cells can undergo repeated self-renewal while retaining genetic integrity, but they occasionally acquire aneuploidy during long-term culture, which is a practical obstacle for medical applications of human pluripotent stem cells. In this study, we explored the biological roles of ABR, a regulator of RHO family small GTPases, and found that it has pivotal roles during mitotic processes in human embryonic stem cells (hESCs). Although ABR has been shown to be involved in dissociation-induced hESC apoptosis, it does not appear to have direct effects on cell survival unless cell-cell contact is impaired. Instead, we found that it is important for faithful hESC division. Mechanistically, ABR depletion compromised centrosome dynamics and predisposed the cell to chromosome misalignment and missegregation, which raised the frequency of aneuploidy. These results provide insights into the mechanisms that support the genetic integrity of self-renewing hESCs.
Rights: © 2017 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/2433/226437
DOI(Published Version): 10.1016/j.stemcr.2017.05.003
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks


Export Format: 


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.