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j.stemcr.2015.07.007.pdf5.74 MBAdobe PDF見る/開く
タイトル: Reprogramming Roadblocks Are System Dependent
著者: Chantzoura, Eleni
Skylaki, Stavroula
Menendez, Sergio
Kim, Shin-Il
Johnsson, Anna
Linnarsson, Sten
Woltjen, Knut
Chambers, Ian
Kaji, Keisuke
発行日: 8-Sep-2015
出版者: Elsevier BV
誌名: Stem Cell Reports
巻: 5
号: 3
開始ページ: 350
終了ページ: 364
抄録: Since the first generation of induced pluripotent stem cells (iPSCs), several reprogramming systems have been used to study its molecular mechanisms. However, the system of choice largely affects the reprogramming efficiency, influencing our view on the mechanisms. Here, we demonstrate that reprogramming triggered by less efficient polycistronic reprogramming cassettes not only highlights mesenchymal-to-epithelial transition (MET) as a roadblock but also faces more severe difficulties to attain a pluripotent state even post-MET. In contrast, more efficient cassettes can reprogram both wild-type and Nanog−/− fibroblasts with comparable efficiencies, routes, and kinetics, unlike the less efficient reprogramming systems. Moreover, we attribute a previously reported variation in the N terminus of KLF4 as a dominant factor underlying these critical differences. Our data establish that some reprogramming roadblocks are system dependent, highlighting the need to pursue mechanistic studies with close attention to the systems to better understand reprogramming.
著作権等: © 2015 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/2433/226438
DOI(出版社版): 10.1016/j.stemcr.2015.07.007
出現コレクション:学術雑誌掲載論文等

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