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Title: Photoresist Micro-Chamber for the Diffracted X-ray Tracking Method Recording Single-Molecule Conformational Changes
Authors: Tahara, Kio
Hirai, Yoshikazu  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-0835-7314 (unconfirmed)
Shimizu, Hirohumi
Tsuchiya, Toshiyuki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7846-5831 (unconfirmed)
Tabata, Osamu
Author's alias: 田原, 樹生
平井, 義和
土屋, 智由
田畑, 修
Keywords: Microfluidics
DXT
diffracted X-ray tracking
Single molecules
Three-deimensional microfabrication
Photoresist
Issue Date: 2016
Publisher: Elsevier BV
Journal title: Procedia Engineering
Volume: 168
Start page: 1394
End page: 1397
Abstract: The diffracted X-ray tracking method has been established for capturing conformational changes of single KcsA potassium channels, which records position of X-ray diffraction spot from a gold nanocrystal attached to the channel. The conventional observation chamber setup could trace a diffraction spot; however relatively high background noise resulting from X-ray scattering degraded a spatial resolution. This paper report a novel low-noise microfabricated observation chamber consisting of SiN membrane window supported by Si and embedded microchannel in negative photoresist. The measured noise exhibited lower level compared to the conventional chamber, which almost reached the detection limit of the employed imaging system.
Description: Proceedings of the 30th anniversary Eurosensors Conference --Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary
Rights: © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/226506
DOI(Published Version): 10.1016/j.proeng.2016.11.389
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