Access count of this item: 191
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
j.proeng.2016.11.389.pdf | 559.54 kB | Adobe PDF | View/Open |
Title: | Photoresist Micro-Chamber for the Diffracted X-ray Tracking Method Recording Single-Molecule Conformational Changes |
Authors: | Tahara, Kio Hirai, Yoshikazu ![]() ![]() ![]() Shimizu, Hirohumi Tsuchiya, Toshiyuki ![]() ![]() ![]() Tabata, Osamu |
Author's alias: | 田原, 樹生 平井, 義和 土屋, 智由 田畑, 修 |
Keywords: | Microfluidics DXT diffracted X-ray tracking Single molecules Three-deimensional microfabrication Photoresist |
Issue Date: | 2016 |
Publisher: | Elsevier BV |
Journal title: | Procedia Engineering |
Volume: | 168 |
Start page: | 1394 |
End page: | 1397 |
Abstract: | The diffracted X-ray tracking method has been established for capturing conformational changes of single KcsA potassium channels, which records position of X-ray diffraction spot from a gold nanocrystal attached to the channel. The conventional observation chamber setup could trace a diffraction spot; however relatively high background noise resulting from X-ray scattering degraded a spatial resolution. This paper report a novel low-noise microfabricated observation chamber consisting of SiN membrane window supported by Si and embedded microchannel in negative photoresist. The measured noise exhibited lower level compared to the conventional chamber, which almost reached the detection limit of the employed imaging system. |
Description: | Proceedings of the 30th anniversary Eurosensors Conference --Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary |
Rights: | © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/226506 |
DOI(Published Version): | 10.1016/j.proeng.2016.11.389 |
Appears in Collections: | Journal Articles |

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.