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タイトル: | Twenty Novel Polymorphic Microsatellite Primers in the Critically Endangered Melastoma tetramerum var. tetramerum (Melastomataceae) |
著者: | Narita, Ayu Izuno, Ayako Komaki, Yoshiteru Tanaka, Takefumi Murata, Jin Isagi, Yuji https://orcid.org/0000-0002-9777-076X (unconfirmed) |
著者名の別形: | 成田, あゆ 伊津野, 彩子 井鷺, 裕司 |
キーワード: | Bonin Islands conservation Melastoma Melastomataceae microsatellites next-generation sequencing |
発行日: | 15-Sep-2016 |
出版者: | Botanical Society of America |
誌名: | Applications in Plant Sciences |
巻: | 4 |
号: | 9 |
論文番号: | 1600053 |
抄録: | Premise of the study: Microsatellite markers were identified for Melastoma tetramerum var. tetramerum (Melastomataceae), a critically endangered shrub endemic to the Bonin Islands, to reveal genetic characteristics in wild and restored populations. Methods and Results: Using next-generation sequencing, 27 microsatellite markers were identified. Twenty of these markers were polymorphic in M. tetramerum var. tetramerum, with two to nine alleles per locus and expected heterozygosity ranging from 0.10 to 0.71. Among the 20 polymorphic markers, 15 were applicable to other closely related taxa, namely M. tetramerum var. pentapetalum, M. candidum var. candidum, and M. candidum var. alessandrense. Conclusions: These markers can be potentially useful to investigate the genetic diversity, population genetic structure, and reproductive ecology of M. tetramerum var. tetramerum as well as of the three related taxa to provide appropriate genetic information for conservation. |
著作権等: | © 2016 Narita et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
URI: | http://hdl.handle.net/2433/227082 |
DOI(出版社版): | 10.3732/apps.1600053 |
PubMed ID: | 27672521 |
出現コレクション: | 学術雑誌掲載論文等 |
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