ダウンロード数: 254
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
s41467-017-00459-x.pdf | 2.23 MB | Adobe PDF | 見る/開く |
タイトル: | Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate |
著者: | Shibata, Tomonori Fujita, Yoshihiko https://orcid.org/0000-0001-6321-2121 (unconfirmed) Ohno, Hirohisa Suzuki, Yuki Hayashi, Karin Komatsu, Kaoru R. Kawasaki, Shunsuke Hidaka, Kumi Yonehara, Shin Sugiyama, Hiroshi https://orcid.org/0000-0001-8923-5946 (unconfirmed) Endo, Masayuki Saito, Hirohide |
著者名の別形: | 柴田, 知範 藤田, 祥彦 大野, 博久 鈴木, 勇輝 林, 香倫 小松, リチャード 馨 川崎, 俊輔 日高, 久美 米原, 伸 杉山, 弘 遠藤, 政幸 齊藤, 博英 |
キーワード: | Nanostructures RNA Synthetic biology |
発行日: | 14-Sep-2017 |
出版者: | Springer Nature |
誌名: | Nature Communications |
巻: | 8 |
号: | 1 |
論文番号: | 540 |
抄録: | Nucleic acid nanotechnology has great potential for future therapeutic applications. However, the construction of nanostructured devices that control cell fate by detecting and amplifying protein signals has remained a challenge. Here we design and build protein-driven RNA-nanostructured devices that actuate in vitro by RNA-binding-protein-inducible conformational change and regulate mammalian cell fate by RNA–protein interaction-mediated protein assembly. The conformation and function of the RNA nanostructures are dynamically controlled by RNA-binding protein signals. The protein-responsive RNA nanodevices are constructed inside cells using RNA-only delivery, which may provide a safe tool for building functional RNA–protein nanostructures. Moreover, the designed RNA scaffolds that control the assembly and oligomerization of apoptosis-regulatory proteins on a nanometre scale selectively kill target cells via specific RNA–protein interactions. These findings suggest that synthetic RNA nanodevices could function as molecular robots that detect signals and localize target proteins, induce RNA conformational changes, and programme mammalian cellular behaviour. |
記述: | 細胞内でタンパク質を検出して運命制御できる「RNAナノマシン」の構築. 京都大学プレスリリース. 2017-09-19. |
著作権等: | © The Author(s) 2017 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
URI: | http://hdl.handle.net/2433/227162 |
DOI(出版社版): | 10.1038/s41467-017-00459-x |
PubMed ID: | 28912471 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-09-19 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。