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Title: Development and Characterization of SSR Markers for Aster savatieri (Asteraceae)
Authors: Ishikawa, Naoko
Sakaguchi, Shota  kyouindb  KAKEN_id  orcid (unconfirmed)
Ito, Motomi
Author's alias: 阪口, 翔太
Keywords: Aster
serpentine plant
Issue Date: Jun-2016
Publisher: Botanical Society of America Inc.
Journal title: Applications in Plant Sciences
Volume: 4
Issue: 6
Thesis number: 1500143
Abstract: Premise of the study: Simple sequence repeat (SSR) markers were developed for Aster savatieri (Asteraceae) and the serpentine variety A. savatieri var. pygmaeus to re-evaluate their taxonomic status. Methods and Results: Using RNA-Seq data, 22 expressed sequence tag (EST)-SSR markers were developed. Polymorphisms were assessed in A. savatieri and in A. savatieri var. pygmaeus. The average number of alleles ranged from four to 15, and expected heterozygosity ranged from 0.417 to 0.870. Transferability was examined in six representative species of Japanese Aster and in Solidago virgaurea subsp. asiatica var. asiatica, a member of the tribe Astereae (Asteraceae); most of the loci were transferable to these examined species. Conclusions: These markers will be useful for genetic studies of variation in A. savatieri and other Aster species that occur in Japan.
Rights: © 2016 Ishikawa et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
DOI(Published Version): 10.3732/apps.1500143
Appears in Collections:Journal Articles

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