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Title: Accurate clinical genetic testing for autoinflammatory diseases using the next-generation sequencing platform MiSeq
Authors: Nakayama, Manabu
Oda, Hirotsugu
Nakagawa, Kenji
Yasumi, Takahiro  kyouindb  KAKEN_id
Kawai, Tomoki
Izawa, Kazushi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-1080-0936 (unconfirmed)
Nishikomori, Ryuta
Heike, Toshio
Ohara, Osamu
Author's alias: 八角, 高裕
河合, 朋樹
井澤, 和司
西小森, 隆太
平家, 俊男
Keywords: Next-generation sequencing (NGS)
Somatic mosaicism
Primary immunodeficiency diseases (PIDs)
Amplicon sequencing
Multiplex PCR
Issue Date: Mar-2017
Publisher: Elsevier BV
Journal title: Biochemistry and Biophysics Reports
Volume: 9
Start page: 146
End page: 152
Abstract: Autoinflammatory diseases occupy one of a group of primary immunodeficiency diseases that are generally thought to be caused by mutation of genes responsible for innate immunity, rather than by acquired immunity. Mutations related to autoinflammatory diseases occur in 12 genes. For example, low-level somatic mosaic NLRP3 mutations underlie chronic infantile neurologic, cutaneous, articular syndrome (CINCA), also known as neonatal-onset multisystem inflammatory disease (NOMID). In current clinical practice, clinical genetic testing plays an important role in providing patients with quick, definite diagnoses. To increase the availability of such testing, low-cost high-throughput gene-analysis systems are required, ones that not only have the sensitivity to detect even low-level somatic mosaic mutations, but also can operate simply in a clinical setting. To this end, we developed a simple method that employs two-step tailed PCR and an NGS system, MiSeq platform, to detect mutations in all coding exons of the 12 genes responsible for autoinflammatory diseases. Using this amplicon sequencing system, we amplified a total of 234 amplicons derived from the 12 genes with multiplex PCR. This was done simultaneously and in one test tube. Each sample was distinguished by an index sequence of second PCR primers following PCR amplification. With our procedure and tips for reducing PCR amplification bias, we were able to analyze 12 genes from 25 clinical samples in one MiSeq run. Moreover, with the certified primers designed by our short program--which detects and avoids common SNPs in gene-specific PCR primers--we used this system for routine genetic testing. Our optimized procedure uses a simple protocol, which can easily be followed by virtually any office medical staff. Because of the small PCR amplification bias, we can analyze simultaneously several clinical DNA samples with low cost and can obtain sufficient read numbers to detect a low level of somatic mosaic mutations.
Rights: © 2016 The Author(s). Published by Elsevier B.V.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
URI: http://hdl.handle.net/2433/227669
DOI(Published Version): 10.1016/j.bbrep.2016.12.002
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