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j.celrep.2017.02.052.pdf4.72 MBAdobe PDF見る/開く
タイトル: Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF5
著者: Obayashi, Eiji
Luna, Rafael E.
Nagata, Takashi  kyouindb  KAKEN_id
Martin-Marcos, Pilar
Hiraishi, Hiroyuki
Singh, Chingakham Ranjit
Erzberger, Jan Peter
Zhang, Fan
Arthanari, Haribabu
Morris, Jacob
Pellarin, Riccardo
Moore, Chelsea
Harmon, Ian
Papadopoulos, Evangelos
Yoshida, Hisashi
Nasr, Mahmoud L.
Unzai, Satoru
Thompson, Brytteny
Aube, Eric
Hustak, Samantha
Stengel, Florian
Dagraca, Eddie
Ananbandam, Asokan
Gao, Philip
Urano, Takeshi
Hinnebusch, Alan G.
Wagner, Gerhard
Asano, Katsura
著者名の別形: 永田, 崇
キーワード: translation initiation
ribosome
eIF5
eIF3
eIF1
start codon selection
ribosomal pre-initiation complex
発行日: 14-Mar-2017
出版者: Elsevier BV
誌名: Cell Reports
巻: 18
号: 11
開始ページ: 2651
終了ページ: 2663
抄録: During eukaryotic translation initiation, eIF3 binds the solvent-accessible side of the 40S ribosome and recruits the gate-keeper protein eIF1 and eIF5 to the decoding center. This is largely mediated by the N-terminal domain (NTD) of eIF3c, which can be divided into three parts: 3c0, 3c1, and 3c2. The N-terminal part, 3c0, binds eIF5 strongly but only weakly to the ribosome-binding surface of eIF1, whereas 3c1 and 3c2 form a stoichiometric complex with eIF1. 3c1 contacts eIF1 through Arg-53 and Leu-96, while 3c2 faces 40S protein uS15/S13, to anchor eIF1 to the scanning pre-initiation complex (PIC). We propose that the 3c0:eIF1 interaction diminishes eIF1 binding to the 40S, whereas 3c0:eIF5 interaction stabilizes the scanning PIC by precluding this inhibitory interaction. Upon start codon recognition, interactions involving eIF5, and ultimately 3c0:eIF1 association, facilitate eIF1 release. Our results reveal intricate molecular interactions within the PIC, programmed for rapid scanning-arrest at the start codon.
著作権等: © 2017 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/2433/227736
DOI(出版社版): 10.1016/j.celrep.2017.02.052
出現コレクション:学術雑誌掲載論文等

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