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Title: Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon
Authors: Nakagawa, Satoshi  kyouindb  KAKEN_id
Saito, Hikari
Tame, Akihiro
Hirai, Miho
Yamaguchi, Hideyuki
Sunata, Takashi
Aida, Masanori
Muto, Hisashi
Sawayama, Shigeki
Takaki, Yoshihiro
Author's alias: 中川, 聡
斉藤, ひかり
武藤, 久
澤山, 茂樹
Keywords: Marine biology
Water microbiology
Issue Date: 18-Aug-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Thesis number: 8764
Abstract: Marine invertebrates associate with diverse microorganisms. Microorganisms even inhabit coelomic fluid (CF), namely, the fluid filling the main body cavity of echinoderms. The CF microbiota potentially impacts host health and disease. Here, we analysed the CF microbiota in two common coastal starfish species, Patiria pectinifera and Asterias amurensis. Although microbial community structures were highly variable among individual starfish, those of P. pectinifera were compositionally similar to those in the surrounding seawater. By contrast, many A. amurensis individuals harboured unique microbes in the CF, which was dominated by the unclassified Thiotrichales or previously unknown Helicobacter-related taxon. In some individuals, the Helicobacter-related taxon was the most abundant genus-level taxon, accounting for up to 97.3% of reads obtained from the CF microbial community. Fluorescence in situ hybridization using a Helicobacter-related-taxon-specific probe suggested that probe-reactive cells in A. amurensis were spiral-shaped, morphologically similar to known Helicobacter species. Electron microscopy revealed that the spiral cells had a prosthecate-like polar appendage that has never been reported in Helicobacter species. Although culture of Helicobacter-related taxon was unsuccessful, this is the first report of the dominance of a Helicobacter-related taxon in invertebrates and non-digestive organs, reshaping our knowledge of the phylogeography of Helicobacter-related taxa.
Rights: © The Author(s) 2017.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
URI: http://hdl.handle.net/2433/228118
DOI(Published Version): 10.1038/s41598-017-09355-2
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