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Title: Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions
Authors: Hörning, Marcel
Nakahata, Masaki
Linke, Philip
Yamamoto, Akihisa  kyouindb  KAKEN_id  orcid (unconfirmed)
Veschgini, Mariam
Kaufmann, Stefan
Takashima, Yoshinori
Harada, Akira
Tanaka, Motomu  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 山本, 暁久
田中, 求
Keywords: Biomaterials-cells
Gels and hydrogels
Issue Date: 9-Aug-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Thesis number: 7660
Abstract: A new class of supramolecular hydrogels, cross-linked by host-guest interactions between β-cyclodextrin (βCD) and adamantane, were designed for the dynamic regulation of cell-substrate interactions. The initial substrate elasticity can be optimized by selecting the molar fraction of host- and guest monomers for the target cells. Moreover, owing to the reversible nature of host-guest interactions, the magnitude of softening and stiffening of the substrate can be modulated by varying the concentrations of free, competing host molecules (βCD) in solutions. By changing the substrate elasticity at a desired time point, it is possible to switch the micromechanical environments of cells. We demonstrated that the Young’s modulus of our “host-guest gels”, 4–11 kPa, lies in an optimal range not only for static (ex situ) but also for dynamic (in situ) regulation of cell morphology and cytoskeletal ordering of myoblasts. Compared to other stimulus-responsive materials that can either change the elasticity only in one direction or rely on less biocompatible stimuli such as UV light and temperature change, our supramolecular hydrogel enables to reversibly apply mechanical cues to various cell types in vitro without interfering cell viability.
Description: An Author Correction to this article was published on 06 March 2018. This article has been updated.
Rights: © The Author(s) 2017.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
DOI(Published Version): 10.1038/s41598-017-07934-x
PubMed ID: 28794475
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