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j.celrep.2017.10.113.pdf | 3.46 MB | Adobe PDF | 見る/開く |
タイトル: | A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs |
著者: | Konagaya, Yumi Terai, Kenta ![]() ![]() Hirao, Yusuke Takakura, Kanako Imajo, Masamichi Kamioka, Yuji Sasaoka, Norio ![]() Kakizuka, Akira ![]() ![]() Sumiyama, Kenta Asano, Tomoichiro Matsuda, Michiyuki ![]() ![]() ![]() |
著者名の別形: | 小長谷, 有美 寺井, 健太 高倉, 加奈子 今城, 正道 笹岡, 紀男 垣塚, 彰 松田, 道行 |
キーワード: | fluorescence resonance energy transfer FRET AMP-activated protein kinase AMPK liver kinase B1 LKB1 peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 Pin1 cell metabolism live imaging |
発行日: | 28-Nov-2017 |
出版者: | Elsevier BV |
誌名: | Cell Reports |
巻: | 21 |
号: | 9 |
開始ページ: | 2628 |
終了ページ: | 2638 |
抄録: | AMP-activated protein kinase (AMPK), a master regulator of cellular metabolism, is a potential target for type 2 diabetes. Although extensive in vitro studies have revealed the complex regulation of AMPK, much remains unknown about the regulation in vivo. We therefore developed transgenic mice expressing a highly sensitive fluorescence resonance energy transfer (FRET)-based biosensor for AMPK, called AMPKAR-EV. AMPKAR-EV allowed us to readily examine the role of LKB1, a canonical stimulator of AMPK, in drug-induced activation and inactivation of AMPK in vitro. In transgenic mice expressing AMPKAR-EV, the AMP analog AICAR activated AMPK in muscle. In contrast, the antidiabetic drug metformin activated AMPK in liver, highlighting the organ-specific action of AMPK stimulators. Moreover, we found that AMPK was activated primarily in fast-twitch muscle fibers after tetanic contraction and exercise. These observations suggest that the AMPKAR-EV mouse will pave a way to understanding the heterogeneous responses of AMPK among cell types in vivo. |
記述: | 生きたマウス体内のAMPK活性を可視化 --糖尿病薬や運動が効果を及ぼす細胞が明らかに--. 京都大学プレスリリース. 2017-11-30. |
著作権等: | © 2017 The Authors. This article is Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0) License. |
URI: | http://hdl.handle.net/2433/228126 |
DOI(出版社版): | 10.1016/j.celrep.2017.10.113 |
PubMed ID: | 29186696 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-11-30-0 |
出現コレクション: | 学術雑誌掲載論文等 |
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