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Title: A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs
Authors: Konagaya, Yumi
Terai, Kenta  kyouindb  KAKEN_id
Hirao, Yusuke
Takakura, Kanako
Imajo, Masamichi
Kamioka, Yuji
Sasaoka, Norio  kyouindb  KAKEN_id
Kakizuka, Akira  kyouindb  KAKEN_id
Sumiyama, Kenta
Asano, Tomoichiro
Matsuda, Michiyuki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-5876-9969 (unconfirmed)
Author's alias: 小長谷, 有美
寺井, 健太
高倉, 加奈子
今城, 正道
笹岡, 紀男
垣塚, 彰
松田, 道行
Keywords: fluorescence resonance energy transfer
FRET
AMP-activated protein kinase
AMPK
liver kinase B1
LKB1
peptidyl-prolyl cis/trans isomerase NIMA-interacting 1
Pin1
cell metabolism
live imaging
Issue Date: 28-Nov-2017
Publisher: Elsevier BV
Journal title: Cell Reports
Volume: 21
Issue: 9
Start page: 2628
End page: 2638
Abstract: AMP-activated protein kinase (AMPK), a master regulator of cellular metabolism, is a potential target for type 2 diabetes. Although extensive in vitro studies have revealed the complex regulation of AMPK, much remains unknown about the regulation in vivo. We therefore developed transgenic mice expressing a highly sensitive fluorescence resonance energy transfer (FRET)-based biosensor for AMPK, called AMPKAR-EV. AMPKAR-EV allowed us to readily examine the role of LKB1, a canonical stimulator of AMPK, in drug-induced activation and inactivation of AMPK in vitro. In transgenic mice expressing AMPKAR-EV, the AMP analog AICAR activated AMPK in muscle. In contrast, the antidiabetic drug metformin activated AMPK in liver, highlighting the organ-specific action of AMPK stimulators. Moreover, we found that AMPK was activated primarily in fast-twitch muscle fibers after tetanic contraction and exercise. These observations suggest that the AMPKAR-EV mouse will pave a way to understanding the heterogeneous responses of AMPK among cell types in vivo.
Description: 生きたマウス体内のAMPK活性を可視化 --糖尿病薬や運動が効果を及ぼす細胞が明らかに--. 京都大学プレスリリース. 2017-11-30.
Rights: © 2017 The Authors.
This article is Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0) License.
URI: http://hdl.handle.net/2433/228126
DOI(Published Version): 10.1016/j.celrep.2017.10.113
PubMed ID: 29186696
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/171129_1.html
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