|Title:||A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs|
Matsuda, Michiyuki https://orcid.org/0000-0002-5876-9969 (unconfirmed)
|Author's alias:||小長谷, 有美|
|Keywords:||fluorescence resonance energy transfer|
AMP-activated protein kinase
liver kinase B1
peptidyl-prolyl cis/trans isomerase NIMA-interacting 1
|Journal title:||Cell Reports|
|Abstract:||AMP-activated protein kinase (AMPK), a master regulator of cellular metabolism, is a potential target for type 2 diabetes. Although extensive in vitro studies have revealed the complex regulation of AMPK, much remains unknown about the regulation in vivo. We therefore developed transgenic mice expressing a highly sensitive fluorescence resonance energy transfer (FRET)-based biosensor for AMPK, called AMPKAR-EV. AMPKAR-EV allowed us to readily examine the role of LKB1, a canonical stimulator of AMPK, in drug-induced activation and inactivation of AMPK in vitro. In transgenic mice expressing AMPKAR-EV, the AMP analog AICAR activated AMPK in muscle. In contrast, the antidiabetic drug metformin activated AMPK in liver, highlighting the organ-specific action of AMPK stimulators. Moreover, we found that AMPK was activated primarily in fast-twitch muscle fibers after tetanic contraction and exercise. These observations suggest that the AMPKAR-EV mouse will pave a way to understanding the heterogeneous responses of AMPK among cell types in vivo.|
|Description:||生きたマウス体内のAMPK活性を可視化 --糖尿病薬や運動が効果を及ぼす細胞が明らかに--. 京都大学プレスリリース. 2017-11-30.|
|Rights:||© 2017 The Authors.|
This article is Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0) License.
|Appears in Collections:||Journal Articles |
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