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Title: High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM
Authors: Keya, Jakia Jannat
Inoue, Daisuke
Suzuki, Yuki
Kozai, Toshiya
Ishikuro, Daiki
Kodera, Noriyuki
Uchihashi, Takayuki
Kabir, Arif Md. Rashedul
Endo, Masayuki  KAKEN_id
Sada, Kazuki
Kakugo, Akira  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 遠藤, 政幸
Keywords: Motor protein function
Issue Date: 21-Jul-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Thesis number: 6166
Abstract: In vitro gliding assay of microtubules (MTs) on kinesins has provided us with valuable biophysical and chemo-mechanical insights of this biomolecular motor system. Visualization of MTs in an in vitro gliding assay has been mainly dependent on optical microscopes, limited resolution of which often render them insufficient sources of desired information. In this work, using high speed atomic force microscopy (HS-AFM), which allows imaging with higher resolution, we monitored MTs and protofilaments (PFs) of tubulins while gliding on kinesins. Moreover, under the HS-AFM, we also observed splitting of gliding MTs into single PFs at their leading ends. The split single PFs interacted with kinesins and exhibited translational motion, but with a slower velocity than the MTs. Our investigation at the molecular level, using the HS-AFM, would provide new insights to the mechanics of MTs in dynamic systems and their interaction with motor proteins.
Rights: © The Author(s) 2017.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
DOI(Published Version): 10.1038/s41598-017-06249-1
PubMed ID: 28733669
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