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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41467-017-02517-w.pdf | 1.28 MB | Adobe PDF | 見る/開く |
| タイトル: | Importance of the 1+7 configuration of ribonucleoprotein complexes for influenza A virus genome packaging |
| 著者: | Noda, Takeshi    https://orcid.org/0000-0002-0658-4663 (unconfirmed)Murakami, Shin Nakatsu, Sumiho Imai, Hirotaka Muramoto, Yukiko https://orcid.org/0000-0002-8706-3113 (unconfirmed)Shindo, Keiko Sagara, Hiroshi Kawaoka, Yoshihiro |
| 著者名の別形: | 野田, 岳志 河岡, 義裕 |
| キーワード: | Electron microscopy Influenza virus Virus structures |
| 発行日: | 4-Jan-2018 |
| 出版者: | Springer Nature |
| 誌名: | Nature Communications |
| 巻: | 9 |
| 論文番号: | 54 |
| 抄録: | The influenza A virus genome is composed of eight single-stranded negative-sense RNAs. Eight distinct viral RNA segments (vRNAs) are selectively packaged into progeny virions, with eight vRNAs in ribonucleoprotein complexes (RNPs) arranged in a specific “1+7” pattern, that is, one central RNP surrounded by seven RNPs. Here we report the genome packaging of an artificially generated seven-segment virus that lacks the hemagglutinin (HA) vRNA. Electron microscopy shows that, even in the presence of only seven vRNAs, the virions efficiently package eight RNPs arranged in the same “1+7” pattern as wild-type virions. Next-generation sequencing reveals that the virions specifically incorporate host-derived 18S and 28S ribosomal RNAs (rRNAs) seemingly as the eighth RNP in place of the HA vRNA. These findings highlight the importance of the assembly of eight RNPs into a specific “1+7” configuration for genome packaging in progeny virions and suggest a potential role for cellular RNAs in viral genome packaging. |
| 記述: | インフルエンザウイルスの遺伝の仕組みを解明. 京都大学プレスリリース. 2018-01-10. |
| 著作権等: | © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/228831 |
| DOI(出版社版): | 10.1038/s41467-017-02517-w |
| PubMed ID: | 29302061 |
| 関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2018-01-10-0 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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