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Title: Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation
Authors: Iraha, Satoshi
Tu, Hung-Ya
Yamasaki, Suguru
Kagawa, Takahiro
Goto, Motohito
Takahashi, Riichi
Watanabe, Takehito
Sugita, Sunao
Yonemura, Shigenobu
Sunagawa, Genshiro A.
Matsuyama, Take
Fujii, Momo
Kuwahara, Atsushi
Kishino, Akiyoshi
Koide, Naoshi
Eiraku, Mototsugu  kyouindb  KAKEN_id
Tanihara, Hidenobu
Takahashi, Masayo
Mandai, Michiko
Keywords: Developmental Biology
Genetics
Cell Biology
Biochemistry
Issue Date: 13-Mar-2018
Publisher: Elsevier BV
Journal title: Stem Cell Reports
Volume: 10
Start page: 1059
End page: 1074
Abstract: Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets.
Rights: © 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/230369
DOI(Published Version): 10.1016/j.stemcr.2018.01.032
PubMed ID: 29503091
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