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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| j.stemcr.2018.01.032.pdf | 7.33 MB | Adobe PDF | 見る/開く |
| タイトル: | Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation |
| 著者: | Iraha, Satoshi Tu, Hung-Ya Yamasaki, Suguru Kagawa, Takahiro Goto, Motohito Takahashi, Riichi Watanabe, Takehito Sugita, Sunao Yonemura, Shigenobu Sunagawa, Genshiro A. Matsuyama, Take Fujii, Momo Kuwahara, Atsushi Kishino, Akiyoshi Koide, Naoshi Eiraku, Mototsugu   Tanihara, Hidenobu Takahashi, Masayo Mandai, Michiko |
| キーワード: | Developmental Biology Genetics Cell Biology Biochemistry |
| 発行日: | 13-Mar-2018 |
| 出版者: | Elsevier BV |
| 誌名: | Stem Cell Reports |
| 巻: | 10 |
| 開始ページ: | 1059 |
| 終了ページ: | 1074 |
| 抄録: | Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets. |
| 著作権等: | © 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| URI: | http://hdl.handle.net/2433/230369 |
| DOI(出版社版): | 10.1016/j.stemcr.2018.01.032 |
| PubMed ID: | 29503091 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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