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タイトル: | Characterisation of an aptamer against the Runt domain of AML1 (RUNX1) by NMR and mutational analyses |
著者: | Takada, Kenta Amano, Ryo Nomura, Yusuke Tanaka, Yoichiro Sugiyama, Shigeru Nagata, Takashi https://orcid.org/0000-0002-3733-2709 (unconfirmed) Katahira, Masato https://orcid.org/0000-0003-0336-7660 (unconfirmed) Nakamura, Yoshikazu Kozu, Tomoko Sakamoto, Traiichi |
著者名の別形: | 永田, 祟 片平, 正人 |
キーワード: | AML1 aptamer Mutation NMR |
発行日: | Feb-2018 |
出版者: | Wiley-Blackwell |
誌名: | FEBS Open Bio |
巻: | 8 |
号: | 2 |
開始ページ: | 264 |
終了ページ: | 270 |
抄録: | Since the invention of systematic evolution of ligands by exponential enrichment, many short oligonucleotides (or aptamers) have been reported that can bind to a wide range of target molecules with high affinity and specificity. Previously, we reported an RNA aptamer that shows high affinity to the Runt domain (RD) of the AML1 protein, a transcription factor with roles in haematopoiesis and immune function. From kinetic and thermodynamic studies, it was suggested that the aptamer recognises a large surface area of the RD, using numerous weak interactions. In this study, we identified the secondary structure by nuclear magnetic resonance spectroscopy and performed a mutational study to reveal the residue critical for binding to the RD. It was suggested that the large contact area was formed by a DNA‐mimicking motif and a multibranched loop, which confers the high affinity and specificity of binding. |
著作権等: | © 2017 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/230576 |
DOI(出版社版): | 10.1002/2211-5463.12368 |
PubMed ID: | 29435416 |
出現コレクション: | 学術雑誌掲載論文等 |
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