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Title: The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses
Authors: Kanai, Yae
Nishihara, Hiroshi
Miyagi, Yohei
Tsuruyama, Tatsuhiro
Taguchi, Kenichi
Katoh, Hiroto
Takeuchi, Tomoyo
Gotoh, Masahiro
Kuramoto, Junko
Arai, Eri
Ojima, Hidenori
Shibuya, Ayako
Yoshida, Teruhiko
Akahane, Toshiaki
Kasajima, Rika
Morita, Kei-ichi
Inazawa, Johji
Sasaki, Takeshi
Fukayama, Masashi
Oda, Yoshinao
Keywords: biobank
empirical analysis
genome research
pathological tissue sample
sample collection and strogage
Issue Date: Feb-2018
Publisher: Wiley-Blackwell
Journal title: Pathology International
Volume: 68
Issue: 2
Start page: 63
End page: 90
Abstract: Genome research using appropriately collected pathological tissue samples is expected to yield breakthroughs in the development of biomarkers and identification of therapeutic targets for diseases such as cancers. In this connection, the Japanese Society of Pathology (JSP) has developed “The JSP Guidelines on the Handling of Pathological Tissue Samples for Genomic Research” based on an abundance of data from empirical analyses of tissue samples collected and stored under various conditions. Tissue samples should be collected from appropriate sites within surgically resected specimens, without disturbing the features on which pathological diagnosis is based, while avoiding bleeding or necrotic foci. They should be collected as soon as possible after resection: at the latest within about 3 h of storage at 4°C. Preferably, snap‐frozen samples should be stored in liquid nitrogen (about −180°C) until use. When intending to use genomic DNA extracted from formalin‐fixed paraffin‐embedded tissue, 10% neutral buffered formalin should be used. Insufficient fixation and overfixation must both be avoided. We hope that pathologists, clinicians, clinical laboratory technicians and biobank operators will come to master the handling of pathological tissue samples based on the standard operating procedures in these Guidelines to yield results that will assist in the realization of genomic medicine.
Rights: © 2018 The Authors. Pathology International published by Japanese Society of Pathology and John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
DOI(Published Version): 10.1111/pin.12631
PubMed ID: 29431262
Appears in Collections:Journal Articles

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