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Title: Single-Molecule Manipulation of the Duplex Formation and Dissociation at the G-Quadruplex/i-Motif Site in the DNA Nanostructure
Authors: M, Endo
X, Xing
X, Zhou
T, Emura
K, Hidaka
B, Tuesuwan
H, Sugiyama
Author's alias: 遠藤, 政幸
江村, 智子
日高, 久美
杉山, 弘
Keywords: DNA origami
high-speed AFM
single-molecule observation
Issue Date: 27-Oct-2015
Publisher: American Chemical Society (ACS)
Journal title: ACS nano
Volume: 9
Issue: 10
Start page: 9922
End page: 9929
Abstract: We demonstrate the single-molecule operation and observation of the formation and resolution of double-stranded DNA (dsDNA) containing a G-quadruplex (GQ) forming and counterpart i-motif forming sequence in the DNA nanostructure. Sequential manipulation of DNA strands in the DNA frame was performed to prepare a topologically controlled GQ/i-motif dsDNA. Using strand displacement and the addition and removal of K+, the topologically controlled GQ/i-motif dsDNA in the DNA frame was obtained in high yield. The dsDNA was resolved into the single-stranded DNA, GQ, and i-motif by the addition of K+ and operation in acidic conditions. The dissociation of the dsDNA under the GQ and i-motif formation condition was monitored by high-speed atomic force microscopy. The results indicate that the dsDNA containing the GQ- and i-motif sequence is effectively dissolved when the duplex is helically loosened in the DNA nanoscaffold.
Rights: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see The full-text file will be made open to the public on 27 October 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1021/acsnano.5b03413
PubMed ID: 26371377
Appears in Collections:Journal Articles

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