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Title: Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions
Authors: Ikeda, Shuntaro  kyouindb  KAKEN_id
Kawahara-Miki, Ryouka
Iwata, Hisataka
Sugimoto, Miki  kyouindb  KAKEN_id
Kume, Shinichi  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 池田, 俊太郎
杉本, 実紀
久米, 新一
Keywords: Animal biotechnology
Issue Date: 19-Jun-2017
Publisher: Springer Nature
Journal title: Scientific reports
Volume: 7
Thesis number: 3800
Abstract: Methionine adenosyltransferase (MAT) is involved in folate-mediated one-carbon metabolism, which is essential for preimplantation embryos in terms of both short-term periconceptional development and long-term phenotypic programming beyond the periconceptional period. Here, our immunofluorescence analysis of bovine oocytes and preimplantation embryos revealed the consistent expression of MAT2A (the catalytic subunit of the ubiquitously expressed-type of MAT isozyme) during this period. Addition of the MAT2A inhibitor FIDAS to the culture media of bovine preimplantation embryos reduced their blastocyst development, revealing the particular importance of MAT2A in successful blastocyst development. Exploration of MAT2A-associated genomic regions in bovine blastocysts using chromatin immunoprecipitation and sequencing (ChIP-seq) identified candidate MAT2A-associated genes implicated not only in short-term periconceptional embryo development, but also in long-term phenotypic programming during this period in terms of growth, metabolism, and immune functions. These results suggest the critical involvement of MAT2A in the periconceptional period in life-long programming of health and disease as well as successful preimplantation development.
Rights: © The Author(s) 2017
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
DOI(Published Version): 10.1038/s41598-017-04003-1
PubMed ID: 28630431
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