このアイテムのアクセス数: 13

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
journal.pone.0195005.pdf17.68 MBAdobe PDF見る/開く
タイトル: BBS1 is involved in retrograde trafficking of ciliary GPCRs in the context of the BBSome complex
著者: Nozaki, Shohei
Katoh, Yohei  kyouindb  KAKEN_id
Kobayashi, Takuya
Nakayama, Kazuhisa  kyouindb  KAKEN_id
著者名の別形: 野崎, 梢平
加藤, 洋平
古林, 拓也
中山, 和久
発行日: 28-Mar-2018
出版者: Public Library of Science (PLoS)
誌名: PloS one
巻: 13
号: 3
論文番号: e0195005
抄録: Protein trafficking within cilia is mediated by the intraflagellar transport (IFT) machinery composed of large protein complexes. The BBSome consists of eight BBS proteins encoded by causative genes of Bardet-Biedl syndrome (BBS), and has been implicated in the trafficking of ciliary membrane proteins, including G protein-coupled receptors (GPCRs), by connecting the IFT machinery to cargo GPCRs. Membrane recruitment of the BBSome to promote cargo trafficking has been proposed to be regulated by the Arf-like small GTPase ARL6/BBS3, through its interaction with the BBS1 subunit of the BBSome. We here investigated how the BBSome core subcomplex composed of BBS1, BBS2, BBS7, and BBS9 assembles and interacts with ARL6, and found that the ARL6–BBS1 interaction is reinforced by BBS9. BBS1-knockout (KO) cells showed defects in the ciliary entry of other BBSome subunits and ARL6, and in ciliary retrograde trafficking and the export of the GPCRs, Smoothened and GPR161. The trafficking defect of these GPCRs was rescued by the exogenous expression of wild-type BBS1, but not by its mutant lacking BBS9-binding ability. Our data thus indicate that the intact BBSome is required for retrograde trafficking of GPCRs out of cilia.
著作権等: © 2018 Nozaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/231082
DOI(出版社版): 10.1371/journal.pone.0195005
PubMed ID: 29590217
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。