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タイトル: | Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice |
著者: | Yamatani, Hiroshi Kohzuma, Kaori https://orcid.org/0000-0003-3899-543X (unconfirmed) Nakano, Michiharu Takami, Tsuneaki Kato, Yusuke Hayashi, Yoriko Monden, Yuki Okumoto, Yutaka Abe, Tomoko Kumamaru, Toshihiro Tanaka, Ayumi Sakamoto, Wataru Kusaba, Makoto |
著者名の別形: | 門田, 有希 奥本, 裕 |
キーワード: | Chlorophyll Lhca4 light-harvesting complex long-term acclimation rice state transition stay-green |
発行日: | 20-Feb-2018 |
出版者: | Oxford University Press (OUP) |
誌名: | Journal of Experimental Botany |
巻: | 69 |
号: | 5 |
開始ページ: | 1027 |
終了ページ: | 1035 |
抄録: | Chlorophyll is an essential molecule for acquiring light energy during photosynthesis. Mutations that result in chlorophyll retention during leaf senescence are called ‘stay-green’ mutants. One of the several types of stay-green mutants, Type E, accumulates high levels of chlorophyll in the pre-senescent leaves, resulting in delayed yellowing. We isolated delayed yellowing1-1 (dye1-1), a rice mutant whose yellowing is delayed in the field. dye1-1 accumulated more chlorophyll than the wild-type in the pre-senescent and senescent leaves, but did not retain leaf functionality in the ‘senescent green leaves’, suggesting that dye1-1 is a Type E stay-green mutant. Positional cloning revealed that DYE1 encodes Lhca4, a subunit of the light-harvesting complex I (LHCI). In dye1-1, amino acid substitution occurs at the location of a highly conserved amino acid residue involved in pigment binding; indeed, a severely impaired structure of the PSI-LHCI super-complex in dye1-1 was observed in a blue native PAGE analysis. Nevertheless, the biomass and carbon assimilation rate of dye1-1 were comparable to those in the wild-type. Interestingly, Lhcb1, a trimeric LHCII protein, was highly accumulated in dye1-1, in the chlorophyll–protein complexes. The high accumulation of LHCII in the LHCI mutant dye1 suggests a novel functional interaction between LHCI and LHCII. |
著作権等: | © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/231098 |
DOI(出版社版): | 10.1093/jxb/erx468 |
PubMed ID: | 29304198 |
出現コレクション: | 学術雑誌掲載論文等 |
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