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タイトル: Post-transcriptional silencing of chalcone synthase is involved in phenotypic lability in petals and leaves of bicolor dahlia (Dahlia variabilis) ‘Yuino’
著者: Ohno, Sho  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-0810-0327 (unconfirmed)
Hori, Wakako
Hosokawa, Munetaka
Tatsuzawa, Fumi
Doi, Motoaki  KAKEN_id
著者名の別形: 大野, 翔
細川, 宗孝
土井, 元章
キーワード: CHS
Flavonoid
Flower color
PTGS
siRNA
発行日: Feb-2018
出版者: Springer Berlin Heidelberg
誌名: Planta
巻: 247
号: 2
開始ページ: 413
終了ページ: 428
抄録: Main conclusion: Post-transcriptional gene silencing (PTGS) of a chalcone synthase ( DvCHS2 ) occurred in the white part of bicolor petals and flavonoid-poor leaves; however, it did not in red petals and flavonoid-rich leaves. Petal color lability is a prominent feature of bicolor dahlia cultivars, and causes plants to produce not only original bicolor petals with colored bases and pure white tips, but also frequently single-colored petals without white tips. In this study, we analysed the molecular mechanisms that are associated with petal color lability using the red-white bicolor cultivar ‘Yuino’. Red single-colored petals lose their white tips as a result of recover of flavonoid biosynthesis. Among flavonoid biosynthetic genes including four chalcone synthase (CHS)-like genes (DvCHS1, DvCHS2, DvCHS3, and DvCHS4), DvCHS1 and DvCHS2 had significantly lower expression levels in the white part of bicolor petals than in red petals, while DvCHS3, DvCHS4, and other flavonoid biosynthetic genes had almost the same expression levels. Small RNAs from the white part of a bicolor petal were mapped onto DvCHS1 and DvCHS2, while small RNAs from a red single-colored petal were not mapped onto any of the four CHS genes. A relationship between petal color and leaf flavonoid accumulation has previously been demonstrated, whereby red petal-producing plants accumulate flavonoids in their leaves, while bicolor petal-producing plants tend not to. The expression level of DvCHS2 was down-regulated in flavonoid-poor leaves and small RNAs from flavonoid-poor leaves were mapped onto DvCHS2, suggesting that the down-regulation of DvCHS2 in flavonoid-poor leaves occurs post-transcriptionally. Genomic analysis also suggested that DvCHS2 is the key gene involved in bicolor formation. Together, these results suggest that post-transcriptional gene silencing of DvCHS2 plays a key role in phenotypic lability in this bicolor dahlia.
著作権等: This is a post-peer-review, pre-copyedit version of an article published in Planta. The final authenticated version is available online at: http://dx.doi.org/.10.1007/s00425-017-2796-3. The full-text file will be made open to the public on 23 October 2018 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/231367
DOI(出版社版): 10.1007/s00425-017-2796-3
PubMed ID: 29063185
出現コレクション:学術雑誌掲載論文等

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