Downloads: 119

Files in This Item:
File Description SizeFormat 
s13059-018-1446-9.pdf5.31 MBAdobe PDFView/Open
Title: SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology
Authors: Abdelmoez, Mahmoud N.
Iida, Kei  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7130-8705 (unconfirmed)
Oguchi, Yusuke
Nishikii, Hidekazu
Yokokawa, Ryuji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-6306-2693 (unconfirmed)
Kotera, Hidetoshi
Uemura, Sotaro
Santiago, Juan G.
Shintaku, Hirofumi
Author's alias: 飯田, 慶
小口, 祐伴
錦井, 秀和
横川, 隆司
小寺, 秀俊
上村, 想太郎
新宅, 博文
Keywords: Single cell
RNA-seq
Microfluidics
RNA transport
Splicing
Isotachophoresis
Nucleus
Cytoplasm
Issue Date: 6-Jun-2018
Publisher: Springer Nature
Journal title: Genome Biology
Volume: 19
Thesis number: 66
Abstract: We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gene expression in the subcellular compartments, and combines them to create novel single-cell RNA-seq data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA and nucRNA that reflect the transient physiological state of single cells. These data provide unique insights into the regulatory network of messenger RNA from the nucleus toward the cytoplasm at the single-cell level.
Description: 1細胞RNA分画解読法の開発に成功 --細胞生物学の研究を加速--. 京都大学プレスリリース. 2018-06-07.
Rights: © The Author(s). 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
URI: http://hdl.handle.net/2433/231406
DOI(Published Version): 10.1186/s13059-018-1446-9
PubMed ID: 29871653
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2018-06-07
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks


Export Format: 


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.