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Title: SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology
Authors: Abdelmoez, Mahmoud N.
Iida, Kei  kyouindb  KAKEN_id  orcid (unconfirmed)
Oguchi, Yusuke
Nishikii, Hidekazu
Yokokawa, Ryuji  kyouindb  KAKEN_id  orcid (unconfirmed)
Kotera, Hidetoshi
Uemura, Sotaro
Santiago, Juan G.
Shintaku, Hirofumi
Author's alias: 飯田, 慶
小口, 祐伴
錦井, 秀和
横川, 隆司
小寺, 秀俊
上村, 想太郎
新宅, 博文
Keywords: Single cell
RNA transport
Issue Date: 6-Jun-2018
Publisher: Springer Nature
Journal title: Genome Biology
Volume: 19
Thesis number: 66
Abstract: We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gene expression in the subcellular compartments, and combines them to create novel single-cell RNA-seq data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA and nucRNA that reflect the transient physiological state of single cells. These data provide unique insights into the regulatory network of messenger RNA from the nucleus toward the cytoplasm at the single-cell level.
Description: 1細胞RNA分画解読法の開発に成功 --細胞生物学の研究を加速--. 京都大学プレスリリース. 2018-06-07.
Rights: © The Author(s). 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.
DOI(Published Version): 10.1186/s13059-018-1446-9
PubMed ID: 29871653
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