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dc.contributor.authorKawabata Galbraith, Kellyen
dc.contributor.authorFujishima, Kazutoen
dc.contributor.authorMizuno, Hiroakien
dc.contributor.authorLee, Sung-Jinen
dc.contributor.authorUemura, Takeshien
dc.contributor.authorSakimura, Kenjien
dc.contributor.authorMishina, Masayoshien
dc.contributor.authorWatanabe, Naokien
dc.contributor.authorKengaku, Minekoen
dc.contributor.alternative川端 Galbraith, ケリーja
dc.contributor.alternative藤島, 和人ja
dc.contributor.alternative水野, 裕昭ja
dc.contributor.alternative李, 聖真ja
dc.contributor.alternative植村, 健ja
dc.contributor.alternative崎村, 建司ja
dc.contributor.alternative三品, 昌美ja
dc.contributor.alternative渡邊, 直樹ja
dc.contributor.alternative見学, 美根子ja
dc.date.accessioned2018-07-17T02:16:15Z-
dc.date.available2018-07-17T02:16:15Z-
dc.date.issued2018-07-03-
dc.identifier.issn2211-1247-
dc.identifier.urihttp://hdl.handle.net/2433/232641-
dc.descriptionアクチン繊維のわずかな偏りが脳神経細胞の形を決めることを発見しました. 京都大学プレスリリース. 2018-07-17.ja
dc.description.abstractDendritic filopodia of developing neurons function as environmental sensors, regulating the spatial organization of dendrites and proper targeting to presynaptic partners. Dendritic filopodia morphology is determined by the balance of F-actin assembled via two major nucleating pathways, the ARP2/3 complex and formins. The inverse-BAR protein MTSS1 is highly expressed in Purkinje cells (PCs) and has been shown to upregulate ARP2/3 activity. PCs in MTSS1 conditional knockout mice showed dendrite hypoplasia due to excessive contact-induced retraction during development. This phenotype was concomitant with elongated dendritic filopodia and was phenocopied by overactivation of the actin nucleator formin DAAM1 localized in the tips of PC dendritic protrusions. Cell biology assays including single-molecule speckle microscopy demonstrated that MTSS1’s C terminus binds to DAAM1 and paused DAAM1-mediated F-actin polymerization. Thus, MTSS1 plays a dual role as a formin inhibitor and ARP2/3 activator in dendritic filopodia, determining final neuronal morphology.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier BVen
dc.rights© 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en
dc.subjectdendriteen
dc.subjectactinen
dc.subjectPurkinje cellen
dc.subjectneuronen
dc.subjectfilopodiaen
dc.subjectspineen
dc.subjectARP2/3en
dc.subjectforminen
dc.subjectMTSS1en
dc.subjectDAAM1en
dc.titleMTSS1 Regulation of Actin-Nucleating Formin DAAM1 in Dendritic Filopodia Determines Final Dendritic Configuration of Purkinje Cellsen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleCell Reportsen
dc.identifier.volume24-
dc.identifier.issue1-
dc.identifier.spage95-
dc.identifier.epage106.e9-
dc.relation.doi10.1016/j.celrep.2018.06.013-
dc.textversionpublisher-
dc.identifier.pmid29972794-
dc.relation.urlhttps://www.kyoto-u.ac.jp/ja/research-news/2018-07-17-
dcterms.accessRightsopen access-
datacite.awardNumber16H06484-
datacite.awardNumber16H04676-
datacite.awardNumber16K18363-
datacite.awardNumber16J11619-
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
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