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タイトル: | DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity |
著者: | Usui, Kenji Okada, Arisa Sakashita, Shungo Shimooka, Masayuki Tsuruoka, Takaaki Nakano, Shu-ichi Miyoshi, Daisuke Mashima, Tsukasa Katahira, Masato https://orcid.org/0000-0003-0336-7660 (unconfirmed) Hamada, Yoshio |
著者名の別形: | 真嶋, 司 片平, 正人 |
キーワード: | designed peptide G-wire G-quadruplex protease peptide nucleic acid PNA |
発行日: | 16-Nov-2017 |
出版者: | MDPI AG |
誌名: | Molecules |
巻: | 22 |
号: | 11 |
論文番号: | 1991 |
抄録: | The development of a switching system for guanine nanowire (G-wire) formation by external signals is important for nanobiotechnological applications. Here, we demonstrate a DNA nanostructural switch (G-wire <--> particles) using a designed peptide and a protease. The peptide consists of a PNA sequence for inducing DNA to form DNA–PNA hybrid G-quadruplex structures, and a protease substrate sequence acting as a switching module that is dependent on the activity of a particular protease. Micro-scale analyses via TEM and AFM showed that G-rich DNA alone forms G-wires in the presence of Ca2+, and that the peptide disrupted this formation, resulting in the formation of particles. The addition of the protease and digestion of the peptide regenerated the G-wires. Macro-scale analyses by DLS, zeta potential, CD, and gel filtration were in agreement with the microscopic observations. These results imply that the secondary structure change (DNA G-quadruplex <--> DNA/PNA hybrid structure) induces a change in the well-formed nanostructure (G-wire <--> particles). Our findings demonstrate a control system for forming DNA G-wire structures dependent on protease activity using designed peptides. Such systems hold promise for regulating the formation of nanowire for various applications, including electronic circuits for use in nanobiotechnologies. |
著作権等: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0). |
URI: | http://hdl.handle.net/2433/233934 |
DOI(出版社版): | 10.3390/molecules22111991 |
PubMed ID: | 29144399 |
出現コレクション: | 学術雑誌掲載論文等 |
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