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Title: In vivo targeted single-nucleotide editing in zebrafish
Authors: Tanaka, Shingo
Yoshioka, Shin
Nishida, Keiji
Hosokawa, Hiroshi
Kakizuka, Akira  kyouindb  KAKEN_id
Maegawa, Shingo  kyouindb  KAKEN_id
Author's alias: 細川, 浩
垣塚, 彰
前川, 真吾
Issue Date: 30-Jul-2018
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 8
Thesis number: 11423
Abstract: To date, several genome editing technologies have been developed and are widely utilized in many fields of biology. Most of these technologies, if not all, use nucleases to create DNA double-strand breaks (DSBs), raising the potential risk of cell death and/or oncogenic transformation. The risks hinder their therapeutic applications in humans. Here, we show that in vivo targeted single-nucleotide editing in zebrafish, a vertebrate model organism, can be successfully accomplished with the Target-AID system, which involves deamination of a targeted cytidine to create a nucleotide substitution from cytosine to thymine after replication. Application of the system to two zebrafish genes, chordin (chd) and one-eyed pinhead (oep), successfully introduced premature stop codons (TAG or TAA) in the targeted genomic loci. The modifications were heritable and faithfully produced phenocopies of well-known homozygous mutants of each gene. These results demonstrate for the first time that the Target-AID system can create heritable nucleotide substitutions in vivo in a programmable manner, in vertebrates, namely zebrafish.
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/234191
DOI(Published Version): 10.1038/s41598-018-29794-9
PubMed ID: 30061715
Appears in Collections:Journal Articles

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