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Title: Silkworms suppress the release of green leaf volatiles by mulberry leaves with an enzyme from their spinnerets
Authors: Takai, Hiroki
Ozawa, Rika
Takabayashi, Junji  kyouindb  KAKEN_id
Fujii, Saki
Arai, Kiriko
Ichiki, Ryoko T.
Koeduka, Takao
Dohra, Hideo
Ohnishi, Toshiyuki
Taketazu, Sakura
Kobayashi, Jun
Kainoh, Yooichi
Nakamura, Satoshi
Fujii, Takeshi
Ishikawa, Yukio
Kiuchi, Takashi
Katsuma, Susumu
Uefune, Masayoshi
Shimada, Toru
Matsui, Kenji
Author's alias: 高井, 嘉樹
小澤, 理香
髙林, 純示
藤井, 沙季
荒井, 紀梨子
一木, 良子
肥塚, 崇男
道羅, 英夫
大西, 利幸
竹田津, 桜
小林, 淳
戒能, 洋一
中村, 達
藤井, 毅
石川, 幸男
木内, 隆史
勝間, 進
上船, 雅義
嶋田, 透
松井, 健二
Keywords: Molecular ecology
Oxidoreductases
Issue Date: 9-Aug-2018
Publisher: Springer Nature America, Inc
Journal title: Scientific Reports
Volume: 8
Thesis number: 11942
Abstract: In response to herbivory, plants emit a blend of volatile organic compounds that includes green leaf volatiles (GLVs) and terpenoids. These volatiles are known to attract natural enemies of herbivores and are therefore considered to function as an indirect defense. Selection should favor herbivores that are able to suppress these volatile emissions, and thereby make themselves less conspicuous to natural enemies. We tested this possibility for silkworms, which were observed to leave secretions from their spinnerets while feeding on mulberry leaves. When we ablated the spinnerets of silkworms, no secretions were observed. Leaves infested by intact silkworms released smaller amounts of GLVs than leaves infested by ablated silkworms, indicating that the spinneret secretion suppressed GLV production. This difference in GLV emissions was also reflected in the behavioral response of Zenillia dolosa (Tachinidae), a parasitoid fly of silkworms. The flies laid fewer eggs when exposed to the volatiles from intact silkworm-infested leaves than when exposed to the volatiles from ablated silkworm-infested leaves. We identified a novel enzyme in the secretion from the spinneret that is responsible for the GLV suppression. The enzyme converted 13(S)-hydroperoxy-(9Z, 11E, 15Z)-octadecatrienoic acid, an intermediate in the biosynthetic pathway of GLVs, into its keto-derivative in a stereospecific manner. Taken together, this study shows that silkworms are able to feed on mulberry in a stealthy manner by suppressing GLV production with an enzyme in secretions of their spinnerets, which might be a countermeasure against induced indirect defense by mulberry plants.
Description: カイコの生き残り戦略を解明 --植物の香り生成を操作する新規酵素発見--. 京都大学プレスリリース. 2018-09-03.
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/234198
DOI(Published Version): 10.1038/s41598-018-30328-6
PubMed ID: 30093702
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2018/180809_4.html
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