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Title: Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)
Authors: Nakai, Risako
Ohnuki, Mari
Kuroki, Kota
Ito, Haruka
Hirai, Hirohisa
Kitajima, Ryunosuke
Fujimoto, Toko
Nakagawa, Masato
Enard, Wolfgang
Imamura, Masanori
Author's alias: 仲井, 理沙子
大貫, 茉里
黒木, 康太
井藤, 晴香
平井, 啓久
北島, 龍之介
藤本, 童子
中川, 誠人
今村, 公紀
Keywords: Evolutionary developmental biology
Issue Date: 15-Aug-2018
Publisher: Springer Nature America, Inc
Journal title: Scientific Reports
Volume: 8
Thesis number: 12187
Abstract: Non-human primates are our closest relatives and are of special interest for ecological, evolutionary and biomedical research. The Japanese macaque (Macaca fuscata) has contributed to the progress of primatology and neurosciences over 60 years. Despite this importance, the molecular and cellular basis of the Japanese macaque remains unexplored since useful cellular tools are lacking. Here we generated induced pluripotent stem cells (iPSCs) from skin fibroblasts of the Japanese macaque with Sendai virus or plasmid vectors. The Japanese macaque iPSCs (jm-iPSCs) were established under feeder-free culture conditions, but feeder cells turned out to be essential for their maintenance. The jm-iPSCs formed human iPSC-like flat colonies which were positive for pluripotent antigens including alkaline phosphatase, SSEA4, and TRA-1-81. They also expressed endogenous OCT3/4, SOX2, L-MYC, and KLF4 and other pluripotent marker genes. The potential to differentiate into all three germ layers and neural stem cells was confirmed by embryoid body and neurosphere formation, respectively. The jm-iPSCs will provide a robust in vitro tool for investigating the underlying mechanisms of development and physiology studies with the Japanese macaque.
Description: 日本固有のサルであるニホンザルのiPS細胞の作製に成功 --iPS細胞を使った霊長類の脳神経科学や進化研究への展開--. 京都大学プレスリリース. 2018-09-06.
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
DOI(Published Version): 10.1038/s41598-018-30734-w
PubMed ID: 30111816
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