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タイトル: Detection of betacyanin in red-tube spinach (Spinacia oleracea) and its biofortification by strategic hydroponics
著者: Watanabe, Sho
Ohtani, Yuta
Aoki, Wataru
Uno, Yuko
Sukekiyo, Yasunori
Kubokawa, Seiichi
Ueda, Mitsuyoshi
著者名の別形: 渡辺, 祥
大谷, 優太
青木, 航
植田, 充美
発行日: 7-Sep-2018
出版者: Public Library of Science (PLoS)
誌名: PLOS ONE
巻: 13
号: 9
論文番号: e0203656
抄録: Betacyanins have been reported as water-soluble, nitrogenous pigments found in the order Caryophyllales, and they are known for powerful natural antioxidant. The biofortification of secondary metabolites such as anthocyanins and betacyanins has recently been performed in food crops by metabolic engineering through genetic modification. However, the distribution of genetically modified foods is strictly regulated. Therefore, we aimed to develop a new method for biofortifying natural antioxidants, betacyanins, without genetic modification. We first detected the presence of betacyanins in red-tube spinach (Spinacia oleracea) through ultraviolet-visible spectroscopy and mass spectrometry. We then hydroponically cultivated plants in the presence of three candidate compounds for betacyanin biofortification: dopamine, Ca2+, and sucrose. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) and antioxidant activity analyses showed that sucrose was most successful in biofortifying betacyanins, and reverse transcription polymerase chain reaction (RT-PCR) indicated that several genes involved in betacyanin biosynthesis were induced by sucrose. Therefore, strategic hydroponics represents a new approach for cultivating betacyanin-enriched vegetables.
記述: 胎児脳発達や抗酸化機能を持つ野菜の開発に成功 --野菜工場へのマルチオミックス解析の展開--. 京都大学プレスリリース. 2018-09-13.
著作権等: © 2018 Watanabe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/234559
DOI(出版社版): 10.1371/journal.pone.0203656
PubMed ID: 30192863
関連リンク: http://www.kyoto-u.ac.jp/ja/research/research_results/2018/180908_2.html
出現コレクション:学術雑誌掲載論文等

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