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タイトル: | Translation of Hepatitis A Virus IRES Is Upregulated by a Hepatic Cell-Specific Factor |
著者: | Sadahiro, Akitoshi Fukao, Akira Kosaka, Mio Funakami, Yoshinori Takizawa, Naoki Takeuchi, Osamu https://orcid.org/0000-0002-1260-6232 (unconfirmed) Duncan, Kent E. Fujiwara, Toshinobu |
著者名の別形: | 貞廣, 暁利 竹内, 理 |
キーワード: | Hepatitis A Virus (HAV) internal ribosome entry site (IRES) translation regulation tropism translation initiation |
発行日: | 10-Aug-2018 |
出版者: | Frontiers Media SA |
誌名: | Frontiers in genetics |
巻: | 9 |
論文番号: | 307 |
抄録: | Many viruses strongly prefer to infect certain cell types, a phenomenon known as “tropism.” Understanding tropism’s molecular basis is important for the design of vaccines and antiviral therapy. A common mechanism involves viral protein interactions with cell-specific surface receptors, but intracellular mechanisms involving translation have also been described. In this report, we focus on Hepatitis A Virus (HAV) tissue tropism from the standpoint of the translational machinery. HAV genomic RNA, like other positive stranded RNA viruses, is devoid of a cap structure and its translation is driven by highly structured RNA sequences termed internal ribosome entry site (IRES) in the 5′ untranslated region (UTR). Unlike most viral IRESs, HAV IRES-mediated translation requires eIF4E and the 3′ end of HAV RNA is polyadenylated. However, the molecular mechanism of HAV IRES-mediated translation initiation remains poorly understood. We analyzed HAV-IRES-mediated translation in a cell-free system derived from either non-hepatic cells (HeLa) or hepatoma cells (Huh-7) that enables investigation of the contribution of the cap and the poly(A) tail. This revealed that HAV IRES-mediated translation activity in hepatoma cell extracts is higher as compared to extracts derived from a non-hepatic line. Our data suggest that HAV IRES-mediated translation is upregulated by a hepatic cell-specific activator in a poly(A) tail-independent manner. |
著作権等: | © 2018 Sadahiro, Fukao, Kosaka, Funakami, Takizawa, Takeuchi, Duncan and Fujiwara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
URI: | http://hdl.handle.net/2433/234930 |
DOI(出版社版): | 10.3389/fgene.2018.00307 |
PubMed ID: | 30147706 |
出現コレクション: | 学術雑誌掲載論文等 |
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