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タイトル: RFTS-dependent negative regulation of Dnmt1 by nucleosome structure and histone tails
著者: Mishima, Yuichi
Brueckner, Laura
Takahashi, Saori
Kawakami, Toru
Arita, Kyohei
Oka, Shota
Otani, Junji
Hojo, Hironobu
Shirakawa, Masahiro
Shinohara, Akira
Watanabe, Mikio
Suetake, Isao
著者名の別形: 白川, 昌宏
キーワード: Dnmt1
histone
modifications
nucleosomes
replication foci targeting sequence
発行日: 16-Oct-2017
出版者: Wiley
誌名: The FEBS Journal
巻: 284
号: 20
開始ページ: 3455
終了ページ: 3469
抄録: DNA methylation in promoter regions represses gene expression and is copied over mitotic divisions by Dnmt1. Dnmt1 activity is regulated by its replication foci targeting sequence (RFTS) domain which masks the catalytic pocket. It has been shown that Dnmt1 activity on unmethylated DNA is inhibited in nucleosome cores. In the present study, we aimed to assess the effect of nuclesome formation on maintenance methylation at single CpG resolution. We show that Dnmt1 fully methylates naked linker DNA in dinucleosomes, whereas maintenance methylation was repressed at all CpG sites in nucleosome core particles. Deletion of RFTS partly released obstruction of Dnmt1 activity in core particles. Histone H3 tail peptides inhibited Dnmt1 in an RFTS‐dependent manner and repression was modulated by acetylation or methylation. We propose a novel function of RFTS to regulate Dnmt1 activity in nucleosomes.
著作権等: © 2017 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/2433/235000
DOI(出版社版): 10.1111/febs.14205
PubMed ID: 28834260
出現コレクション:学術雑誌掲載論文等

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