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Title: Pantothenate auxotrophy of Methylobacterium spp. isolated from living plants
Authors: Yoshida, Yusuke
Iguchi, Hiroyuki
Sakai, Yasuyoshi  kyouindb  KAKEN_id
Yurimoto, Hiroya  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 吉田, 裕介
由里本, 博也
Keywords: B vitamin auxotrophy
Issue Date: 26-Nov-2018
Publisher: Informa UK Limited
Journal title: Bioscience, biotechnology, and biochemistry
Thesis number: 1549935
Abstract: A number of pink-pigmented facultative methylotrophs (PPFMs) belonging to Methylobacterium spp. isolated from living plant samples were found to require B vitamins for their growth in minimal medium, and most B vitamin-auxotrophic PPFMs required pantothenate (vitamin B5). Further investigation of pantothenate auxotrophy using the representative strain Methylobacterium sp. OR01 demonstrated that this strain cannot synthesize β-alanine, one of the precursors of pantothenate. β-alanine and several precursors of pantothenate restored the growth of Methylobacterium sp. OR01 in minimal medium. Furthermore, this strain could colonize leaves of Arabidopsis thaliana cultivated in medium without pantothenate or its precursors. Pantothenate, β-alanine and several precursors were detected in the suspension of A. thaliana leaves. These results suggest that pantothenate-auxotrophic PPFMs can symbiotically colonize the surface of plant leaves by acquiring β-alanine and other precursors, in addition to pantothenate. Finally, the fitness advantage of B vitamin auxotrophy of PPFMs in the phyllosphere environment is discussed. Pantothenate-auxotrophic strains of Methylobacterium show symbiotic colonization on plants through acquisition of pantothenate and its precursors.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, biotechnology, and biochemistry on 26 November 2018, available online:
The full-text file will be made open to the public on 26 November 2019 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1080/09168451.2018.1549935
PubMed ID: 30475153
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