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Title: Ultimate downsizing of D-fructose dehydrogenase for improving the performance of direct electron transfer-type bioelectrocatalysis
Authors: Kaida, Yuya
Hibino, Yuya
Kitazumi, Yuki
Shirai, Osamu
Kano, Kenji
Author's alias: 戒田, 優也
日比野, 佑哉
北隅, 優希
白井, 理
加納, 健司
Keywords: Fructose dehydrogenase
Direct electron transfer
Protein engineering
Downsizing
Redox potential shift
Issue Date: Jan-2019
Publisher: Elsevier Inc.
Journal title: Electrochemistry Communications
Volume: 98
Start page: 101
End page: 105
Abstract: d-Fructose dehydrogenase (FDH), a membrane-bound heterotrimeric enzyme, shows strong activity in direct electron transfer (DET)-type bioelectrocatalysis. An FDH variant (Δ1c2cFDH) which lacks 199 amino acid residues including two heme c moieties from N-terminus was constructed, and its DET-type bioelectrocatalytic performance was evaluated with cyclic voltammetry at Au planar electrodes. A DET-type catalytic current of d-fructose oxidation was clearly observed on Δ1c2cFDH-adsorbed Au electrodes. Detailed analysis of the steady-state catalytic current indicated that Δ1c2cFDH transports the electrons to the electrode via heme 3c at a more negative potential and at more improved kinetics than the recombinant (native) FDH.
Rights: © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
URI: http://hdl.handle.net/2433/235717
DOI(Published Version): 10.1016/j.elecom.2018.12.001
Appears in Collections:Journal Articles

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