ダウンロード数: 339
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.kint.2018.10.017.pdf | 7.11 MB | Adobe PDF | 見る/開く |
タイトル: | Myofibroblasts acquire retinoic acid–producing ability during fibroblast-to-myofibroblast transition following kidney injury |
著者: | Nakamura, Jin Sato, Yuki Kitai, Yuichiro Wajima, Shuichi Yamamoto, Shinya Oguchi, Akiko Yamada, Ryo https://orcid.org/0000-0002-1587-630X (unconfirmed) Kaneko, Keiichi Kondo, Makiko Uchino, Eiichiro Tsuchida, Junichi Hirano, Keita https://orcid.org/0000-0003-3695-9056 (unconfirmed) Sharma, Kumar Kohno, Kenji Yanagita, Motoko |
著者名の別形: | 中村, 仁 佐藤, 有紀 北井, 悠一朗 小口, 綾貴子 近藤, 麻紀子 内野, 詠一郎 土田, 潤一 比良野, 圭太 河野, 憲二 柳田, 素子 |
キーワード: | chronic kidney disease fibroblast fibrosis proximal tubule |
発行日: | Mar-2019 |
出版者: | Elsevier BV |
誌名: | Kidney International |
巻: | 95 |
号: | 3 |
開始ページ: | 526 |
終了ページ: | 539 |
抄録: | Tubular injury and interstitial fibrosis are the hallmarks of chronic kidney disease. While recent studies have verified that proximal tubular injury triggers interstitial fibrosis, the impact of fibrosis on tubular injury and regeneration remains poorly understood. We generated a novel mouse model expressing diphtheria toxin receptor on renal fibroblasts to allow for the selective disruption of renal fibroblast function. Administration of diphtheria toxin induced upregulation of the tubular injury marker Ngal and caused tubular proliferation in healthy kidneys, whereas administration of diphtheria toxin attenuated tubular regeneration in fibrotic kidneys. Microarray analysis revealed down-regulation of the retinol biosynthesis pathway in diphtheria toxin-treated kidneys. Healthy proximal tubules expressed retinaldehyde dehydrogenase 2 (RALDH2), a rate-limiting enzyme in retinoic acid biosynthesis. After injury, proximal tubules lost RALDH2 expression, whereas renal fibroblasts acquired strong expression of RALDH2 during the transition to myofibroblasts in several models of kidney injury. The retinoic acid receptor (RAR) RARγ was expressed in proximal tubules both with and without injury, and αB-crystallin, the product of an RAR target gene, was strongly expressed in proximal tubules after injury. Furthermore, BMS493, an inverse agonist of RARs, significantly attenuated tubular proliferation in vitro. In human biopsy tissue from patients with IgA nephropathy, detection of RALDH2 in the interstitium correlated with older age and lower kidney function. These results suggest a role of retinoic acid signaling and cross-talk between fibroblasts and tubular epithelial cells during tubular injury and regeneration, and may suggest a beneficial effect of fibrosis in the early response to injury. |
記述: | 腎障害における線維化の正の側面の発見 --線維化が腎臓を修復する--. 京都大学プレスリリース. 2019-01-18. |
著作権等: | © 2019, International Society of Nephrology. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/236048 |
DOI(出版社版): | 10.1016/j.kint.2018.10.017 |
PubMed ID: | 30661714 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2019-01-18-0 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。