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タイトル: Inner lumen proteins stabilize doublet microtubules in cilia and flagella
著者: Owa, Mikito
Uchihashi, Takayuki
Yanagisawa, Haru-aki
Yamano, Takashi  kyouindb  KAKEN_id
Iguchi, Hiro
Fukuzawa, Hideya
Wakabayashi, Ken-ichi
Ando, Toshio
Kikkawa, Masahide
著者名の別形: 大和, 幹人
内橋, 貴之
柳澤, 春明
山野, 隆志
井口, ひろ
福澤, 秀哉
若林, 憲一
安藤, 敏夫
吉川, 雅英
キーワード: Atomic force microscopy
Cryoelectron tomography
発行日: 8-Mar-2019
出版者: Springer Nature
誌名: Nature Communications
巻: 10
論文番号: 1143
抄録: Motile cilia are microtubule-based organelles that play important roles in most eukaryotes. Although axonemal microtubules are sufficiently stable to withstand their beating motion, it remains unknown how they are stabilized while serving as tracks for axonemal dyneins. To address this question, we have identified two uncharacterized proteins, FAP45 and FAP52, as microtubule inner proteins (MIPs) in Chlamydomonas. These proteins are conserved among eukaryotes with motile cilia. Using cryo-electron tomography (cryo-ET) and high-speed atomic force microscopy (HS-AFM), we show that lack of these proteins leads to a loss of inner protrusions in B-tubules and less stable microtubules. These protrusions are located near the inner junctions of doublet microtubules and lack of both FAP52 and a known inner junction protein FAP20 results in detachment of the B-tubule from the A-tubule, as well as flagellar shortening. These results demonstrate that FAP45 and FAP52 bind to the inside of microtubules and stabilize ciliary axonemes.
著作権等: © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/237314
DOI(出版社版): 10.1038/s41467-019-09051-x
PubMed ID: 30850601
出現コレクション:学術雑誌掲載論文等

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