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dc.contributor.authorGunawan, Felix
dc.contributor.authorGentile, Alessandra
dc.contributor.authorFukuda, Ryuichi
dc.contributor.authorTsedeke, Ayele Taddese
dc.contributor.authorJiménez-Amilburu, Vanesa
dc.contributor.authorRamadass, Radhan
dc.contributor.authorIida, Atsuo
dc.contributor.authorSehara-Fujisawa, Atsuko
dc.contributor.authorStainier, Didier Y.R.
dc.contributor.alternative飯田, 敦夫
dc.contributor.alternative瀬原, 淳子
dc.date.accessioned2019-03-22T01:51:16Z-
dc.date.available2019-03-22T01:51:16Z-
dc.date.issued2019-03-04
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/2433/237392-
dc.description.abstractElucidating the morphogenetic events that shape vertebrate heart valves, complex structures that prevent retrograde blood flow, is critical to understanding valvular development and aberrations. Here, we used the zebrafish atrioventricular (AV) valve to investigate these events in real time and at single-cell resolution. We report the initial events of collective migration of AV endocardial cells (ECs) into the extracellular matrix (ECM), and their subsequent rearrangements to form the leaflets. We functionally characterize integrin-based focal adhesions (FAs), critical mediators of cell–ECM interactions, during valve morphogenesis. Using transgenes to block FA signaling specifically in AV ECs as well as loss-of-function approaches, we show that FA signaling mediated by Integrin α5β1 and Talin1 promotes AV EC migration and overall shaping of the valve leaflets. Altogether, our investigation reveals the critical processes driving cardiac valve morphogenesis in vivo and establishes the zebrafish AV valve as a vertebrate model to study FA-regulated tissue morphogenesis.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherRockefeller University Press
dc.rights© 2019 Gunawan et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
dc.titleFocal adhesions are essential to drive zebrafish heart valve morphogenesis
dc.type.niitypeJournal Article
dc.identifier.jtitleJournal of Cell Biology
dc.identifier.volume218
dc.identifier.issue3
dc.identifier.spage1039
dc.identifier.epage1054
dc.relation.doi10.1083/jcb.201807175
dc.textversionpublisher
dc.addressDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research
dc.addressDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research
dc.addressDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research
dc.addressDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research
dc.addressDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research
dc.addressDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research
dc.addressInstitute for Frontier Life and Medical Sciences, Kyoto University
dc.addressInstitute for Frontier Life and Medical Sciences, Kyoto University
dc.addressDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research
dc.identifier.pmid30635353
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