|Title:||TRPV4 is functionally expressed in oligodendrocyte precursor cells and increases their proliferation|
Miyake, Takahito https://orcid.org/0000-0002-4356-5883 (unconfirmed)
Shirakawa, Hisashi https://orcid.org/0000-0002-4129-0978 (unconfirmed)
Kaneko, Shuji https://orcid.org/0000-0001-5152-5809 (unconfirmed)
|Author's alias:||大橋, 佳奈|
Oligodendrocyte precursor cells
Protein kinase C
|Journal title:||Pflügers Archiv - European Journal of Physiology|
|Abstract:||Oligodendrocytes, which differentiate from oligodendrocyte precursor cells (OPCs), ensheath axons with myelin, play an essential role in rapid conduction of action potentials and metabolically support neurons. Elucidation of the mechanisms underlying the proliferation, migration, differentiation, and survival of OPCs is considered indispensable for determining the causes of central nervous system diseases. However, the relationship between these functions of OPCs and their intracellular Ca²⁺ signaling has not been fully elucidated. Here, we investigated the function of transient receptor potential vanilloid 4 (TRPV4), a Ca²⁺-permeable channel that responds to hypo-osmolarity, mild temperature, mechanical stimulation, and endogenous arachidonic acid metabolites, in OPCs. Trpv4 mRNA was detected in OPCs in vivo and in primary cultured rat OPCs. In Ca²⁺ imaging experiments, treatment with the selective TRPV4 agonist GSK1016790A induced sustained elevation of the intracellular Ca²⁺ concentration in OPCs in a concentration-dependent manner, which was almost completely suppressed by co-treatment with the selective TRPV4 antagonist HC067047. Stimulation of TRPV4 by GSK1016790A augmented OPC proliferation, which was abolished by co-treatment with HC067047, the intracellular Ca²⁺ chelator BAPTA-AM, and the protein kinase C inhibitor bisindolylmaleimide II. By contrast, GSK1016790A did not significantly affect the migration or differentiation of OPCs. Taken together, these results suggest that TRPV4 is functionally expressed in OPCs and increases the proliferation of these cells without affecting their ability to differentiate into oligodendrocytes.|
|Description:||This article is part of the special issue on Thermal biology in Pflügers Archiv – European Journal of Physiology.|
|Rights:||This is a post-peer-review, pre-copyedit version of an article published in Pflügers Archiv - European Journal of Physiology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00424-018-2130-3.|
This is not the published version. Please cite only the published version.
|Appears in Collections:||Journal Articles|
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